Título:	Characterization and phylogenetic affiliation of Actinobacteria from tropical soils with potential uses for agro-industrial processes.
Autoria:	DORNELAS, J. C. M. FIGUEIREDO, J. E. F. ABREU, C. S. de LANA, U. G. P. OLIVEIRA-PAIVA, C. A. MARRIEL, I. E.
Afiliação:	Universidade Federal de São João Del Rei JOSE EDSON FONTES FIGUEIREDO, CNPMS Universidade Federal de Minas Gerais UBIRACI GOMES DE PAULA LANA, CNPMS CHRISTIANE ABREU DE OLIVEIRA PAIVA, CNPMS IVANILDO EVODIO MARRIEL, CNPMS.
Ano de publicação:	2017
Referência:	Genetics and Molecular Research, Ribeirão Preto, v. 16, n. 3, p. 1-16, 2017.
Conteúdo:	Secondary metabolites produced by Actinobacteria of tropical soils represent a largely understudied source of novel molecules with relevant application in medicine, pharmaceutical and food industries, agriculture, and environmental bioremediation. The present study aimed to characterize sixty-nine Actinobacteria isolated from compost and tropical soils using morphological, biochemical, and molecular methods. All the isolates showed high variation for morphological traits considering the color of pigments of the aerial and vegetative mycelium and spore chain morphology. The enzymatic activity of amylase, cellulase, and lipase was highly variable. The amylase activity was detected in 53 (76.81%) isolates. Eighteen (Streptomyces curacoi) and ACSL 6 (S. hygroscopicus) showed the highest EI values (6.44 and 6.42, respectively). The cellulase activity varied significantly (P ≤ 0.05) among the isolates. Twenty-nine isolates (42.02%) showed high cellulase activity, and the isolates ACJ 48 (S. chiangmaiensis) and ACJ 53 (S. cyslabdanicus) showed the highest EI values (6.56 for both isolates). The lipase activity varied statistically (P ≤ 0.05) with fourteen isolates (20.29%) considered good lipase producers (EI > 2.0). The isolate ACSL 6 (S. hygroscopicus) showed the highest EI value of 2.60. Molecular analysis of partial 16S rRNA gene sequencing revealed the existence of 49 species, being 38 species with only one representative member and 11 species represented by one or more strains. All species belonged to three genera, namely Streptomyces (82.61%), Amycolatopsis (7.25%), and Kitasatospora (10.14%). The present results showed the high biotechnological potential of different Actinobacteria from tropical soils.
Thesagro:	Actinomiceto Atividade enzimática
NAL Thesaurus:	Actinobacteria Enzyme activity
Palavras-chave:	Morphological traits Identificação molecular Molecular identification
Digital Object Identifier:	10.4238/gmr16039703
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPMS)