Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1076282
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dc.contributor.authorPEREIRA, W. J.
dc.contributor.authorBASSINELLO, P. Z.
dc.contributor.authorBRONDANI, C.
dc.contributor.authorVIANELLO, R. P.
dc.date.accessioned2017-09-27T10:32:00Z-
dc.date.available2017-09-27T10:32:00Z-
dc.date.created2017-09-26
dc.date.issued2017
dc.identifier.citationCrop Breeding and Applied Biotechnology, Viçosa, MG, v. 17, n. 2, p. 150-158, abr./jun. 2017.
dc.identifier.issn1518-7853
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1076282-
dc.descriptionAn RNA extraction method with high integrity and purity as well as the selection of adequate reference genes are prerequisites for gene expression analysis. For common bean seeds, there is no well-defined protocol that can be used in a laboratory routine for gene expression analysis. In this study, an extraction protocol for RNA from common bean seeds, which produced material with good integrity for qPCR (RIN ≥ 6.5), was optimized. In addition, 10 reference genes were evaluated under qPCR standard conditions using different tissue samples of common beans. Gene stabilities were analyzed using the delta-CT method, Bestkeeper, NormFinder and geNorm approaches. The genes β-tubulin and T197 were ranked as the most stable among the sample sets evaluated with different tissue samples, while PvAct and Pv18S were the least stable. To our knowledge, this is the first study evaluating RNA isolation methods and reference gene selection for seeds of Phaseolus vulgaris.
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectGene normalization
dc.subjectMolecular breeding
dc.subjectGrain RNA extraction
dc.titleAn improved method for RNA extraction from common bean seeds and validation of reference genes for qPCR.
dc.typeArtigo de periódico
dc.date.updated2017-12-14T11:11:11Zpt_BR
dc.subject.thesagroFeijão
dc.subject.thesagroPhaseolus vulgaris
dc.subject.thesagroMelhoramento
dc.subject.thesagroRNA
dc.subject.nalthesaurusBeans
dc.subject.nalthesaurusGene expression
riaa.ainfo.id1076282
riaa.ainfo.lastupdate2017-12-14 -02:00:00
dc.identifier.doi10.1590/1984-70332017v17n2a22
dc.contributor.institutionWENDELL JACINTO PEREIRA, UFG; PRISCILA ZACZUK BASSINELLO, CNPAF; CLAUDIO BRONDANI, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF.
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