Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080581
Registro completo de metadados
Campo DCValorIdioma
dc.contributor.authorPINTO, I. S. B.
dc.contributor.authorFONSECA, I.
dc.contributor.authorBRANDAO, H. de M.
dc.contributor.authorGERN, J. C.
dc.contributor.authorGUIMARAES, A. S.
dc.contributor.authorCARVALHO, W. A.
dc.contributor.authorBRITO, M. A. V. P. e
dc.contributor.authorVICCINI, L. F.
dc.contributor.authorMARTINS, M. F.
dc.date.accessioned2017-11-23T23:20:22Z-
dc.date.available2017-11-23T23:20:22Z-
dc.date.created2017-11-23
dc.date.issued2017
dc.identifier.citationGenetic and Molecular Research, v. 16, n. 1, 2017.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1080581-
dc.descriptionAbstract Intramammary infections are one of the main causes of productivity loss in dairy cows. To better understand the immune system response and to avoid the use of live animals, we validated the use of isolated bovine udder as an ex situ model. Six mammary glands were collected from cows ready for culling. Three udders were perfused with Tyrode's solution and three were not-perfused. During six hours, we collected perfusate samples for biochemical analysis. We also collected alveolar and teat canal tissue to evaluate gene expression. The biochemical parameters indicated that the perfused udders remained viable for the entire period of the experiment. A real-time polymerase chain reaction showed an increase in 18S rRNA gene expression in the alveolar tissue at 3 and 4 h after perfusion. There was also an increase in the Ubiquitin gene in the teat canal from not-perfused udders at 1, 3, and 4 h after slaughter. In general, gene expression was stable during the experiment. Our results indicated that the isolated perfused bovine udder model is appropriate for genetic studies, opening a new perspective in animal experimentation methods.
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectEx-situ model
dc.subjectMammary gland
dc.titleSHORT-COMMUNICATION: Evaluation of perfused bovine udder for gene expression studies in dairy cows.
dc.typeArtigo de periódico
dc.date.updated2017-12-12T11:11:11Zpt_BR
dc.subject.nalthesaurusgenespt_BR
dc.format.extent27 p.
riaa.ainfo.id1080581
riaa.ainfo.lastupdate2017-12-12 -02:00:00
dc.identifier.doi10.4238/gmr16019637
dc.contributor.institutionISABELLA S. B. PINTO, CNPq; ISABELA FONSECA, CNPq; HUMBERTO DE MELLO BRANDAO, CNPGL; JULIANA CARINE GERN, CNPGL; ALESSANDRO DE SA GUIMARAES, CNPGL; WANESSA ARAUJO CARVALHO, CNPGL; MARIA APARECIDA V PAIVA E BRITO, CNPGL; LYDERSON FACIO VICCINI, UFJF; MARTA FONSECA MARTINS, CNPGL.
Aparece nas coleções:Nota Técnica/Nota científica (CNPGL)

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
Cnpgl2017ShortCommGMREvaluation.pdf553,85 kBAdobe PDFThumbnail
Visualizar/Abrir
Mostrar registro simples do item Visualizar estatísticas

FacebookTwitterDeliciousLinkedInGoogle BookmarksMySpace