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Campo DCValorLengua/Idioma
dc.contributor.authorFIGUEIREDO, M. A. de
dc.contributor.authorCOELHO, S. V. B.
dc.contributor.authorROSA, S. D. V. F. da
dc.contributor.authorVILELA, A. L.
dc.contributor.authorSILVA, L. C.
dc.date.accessioned2017-12-08T23:27:35Z-
dc.date.available2017-12-08T23:27:35Z-
dc.date.created2017-12-08
dc.date.issued2017
dc.identifier.citationJournal of Seed Science, v. 39, n. 2, p. 150-158, 2017.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1081992-
dc.descriptionCryopreservation is a viable option for conservation of coffee germplasm. However, for this technique to be completely successful, it is of fundamental importance to carry out studies that ensure maintenance of cell integrity before and after immersion in liquid nitrogen (LN). Therefore, the aim in this study was to investigate the water content, cooling rate, and final temperature most suitable for cryopreservation of Coffea arabica L. seeds. The seeds were dried by silica gel to water contents of 5, 10, 15, 20, 30 and 40 % wb, subjected to slow cooling treatments at speeds of -1, -3 and -5 °C min.-1 to final temperatures of -40, -50 and -60 °C and then directly immersed in LN. After storage, seeds were rewarmed at 40 °C for two minutes. The survival rate and viability of the seeds and embryos were evaluated by the tetrazolium and germination tests. Results of the tetrazolium test indicate that embryos excised from cryopreserved seeds are less sensitive to cryopreservation than whole seeds are. The water content of 20% wb and the use of zygotic embryos led to the highest survival rate of the coffee seeds, depending on the cooling rate and the final temperature of precooling.
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleExploratory studies for cryopreservation of Coffea arabica L. seeds.
dc.typeArtigo de periódico
dc.date.updated2017-12-08T23:27:35Zpt_BR
dc.subject.thesagroCoffea Arábica
dc.subject.thesagroResfriamentoeng
dc.subject.thesagroConservaçãoeng
dc.subject.thesagroGermoplasmaeng
dc.subject.nalthesaurusWater content
dc.subject.nalthesaurussilica gel
dc.subject.nalthesaurusCryopreservationeng
dc.description.notesTítulo em português: Estudos exploratórios para a criopreservação de sementes de Coffea arabica L.
riaa.ainfo.id1081992
riaa.ainfo.lastupdate2017-12-08
dc.identifier.doihttps://doi.org/10.1590/2317-1545v39n2171123eng
dc.contributor.institutionMADELEINE ALVES DE FIGUEIREDO, UNIVERSIDADE FEDERAL DE LAVRAS
dc.contributor.institutionSTEFANIA VILAS BOAS COELHO, UNIVERSIDADE FEDERAL DE LAVRASeng
dc.contributor.institutionSTTELA DELLYZETE VEIGA F DA ROSA, SAPCeng
dc.contributor.institutionAMANDA LIMA VILELA, UNIVERSIDADE FEDERAL DE LAVRASeng
dc.contributor.institutionLUCIANO COUTINHO SILVA, UNIVERSIDADE FEDERAL DA PARAÍBA.eng
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