Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1082111
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dc.contributor.authorNAKAYAMA, T. J.
dc.contributor.authorRODRIGUES, F. A.
dc.contributor.authorNEUMAIER, N.
dc.contributor.authorGOMES, J. M.
dc.contributor.authorMOLINARI, H. B. C.
dc.contributor.authorSANTIAGO, T. R.
dc.contributor.authorFORMIGHIERI, E. F.
dc.contributor.authorBASSO, M. F.
dc.contributor.authorFARIAS, J. R. B.
dc.contributor.authorEMYGDIO, B. M.
dc.contributor.authorOLIVEIRA, A. C. B. de
dc.contributor.authorCAMPOS, A. D.
dc.contributor.authorBORÉM, A.
dc.contributor.authorHARMON, F. G.
dc.contributor.authorMERTZ-HENNING, L. M.
dc.contributor.authorNEPOMUCENO, A. L.
dc.date.accessioned2017-12-11T23:30:46Z-
dc.date.available2017-12-11T23:30:46Z-
dc.date.created2017-12-11
dc.date.issued2017
dc.identifier.citationPLoS ONE, v. 12, n. 11, e0187920, 2017.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1082111-
dc.descriptionSoybean (Glycine max) is one of the major crops worldwide and flooding stress affects the production and expansion of cultivated areas. Oxygen is essential for mitochondrial aerobic respiration to supply the energy demand of plant cells. Because oxygen diffusion in water is 10,000 times lower than in air, partial (hypoxic) or total (anoxic) oxygen deficiency is important component of flooding. Even when oxygen is externally available, oxygen deficiency frequently occurs in bulky, dense or metabolically active tissues such as phloem, meristems, seeds, and fruits. In this study, we analyzed conserved and divergent root transcriptional responses between flood-tolerant Embrapa 45 and flood-sensitive BR 4 soybean cultivars under hypoxic stress conditions with RNA-seq. To understand how soybean genes evolve and respond to hypoxia, stable and differentially expressed genes were characterized structurally and compositionally comparing its mechanistic relationship. Between cultivars, Embrapa 45 showed less up- and more down-regulated genes, and stronger induction of phosphoglucomutase (Glyma05g34790), unknown protein related to N-terminal protein myristoylation (Glyma06g03430), protein suppressor of phyA-105 (Glyma06g37080), and fibrillin (Glyma10g32620). RNA-seq and qRT-PCR analysis of non-symbiotic hemoglobin (Glyma11g12980) indicated divergence in gene structure between cultivars. Transcriptional changes for genes in amino acids and derivative metabolic process suggest involvement of amino acids metabolism in tRNA modifications, translation accuracy/efficiency, and endoplasmic reticulum stress in both cultivars under hypoxia. Gene groups differed in promoter TATA box, ABREs (ABA-responsive elements), and CRT/DREs (C-repeat/dehydration-responsive elements) frequency. Gene groups also differed in structure, composition, and codon usage, indicating biological significances. Additional data suggests that cis-acting ABRE elements can mediate gene expression independent of ABA in soybean roots under hypoxia.
dc.language.isoporpt_BR
dc.rightsopenAccesspt_BR
dc.subjectHipóxia
dc.subjectInoxiapt_BR
dc.titleInsights into soybean transcriptome reconfiguration under hypoxic stress: Functional, regulatory, structural, and compositional characterization.
dc.typeArtigo de periódico
dc.date.updated2018-01-10T11:11:11Zpt_BR
dc.subject.thesagroDeficiênciapt_BR
dc.subject.thesagroOxigêniopt_BR
dc.subject.thesagroSojapt_BR
dc.subject.nalthesaurusSoybeanspt_BR
dc.subject.nalthesaurusHypoxiapt_BR
riaa.ainfo.id1082111
riaa.ainfo.lastupdate2018-01-10 -02:00:00
dc.identifier.doi10.1371/journal.pone.0187920
dc.contributor.institutionTHIAGO J. NAKAYAMA, UFV; FABIANA A. RODRIGUES; NORMAN NEUMAIER, CNPSO; JULIANA MARCOLINO GOMES; HUGO BRUNO CORREA MOLINARI, CNPAE; THAÍS R. SANTIAGO; EDUARDO FERNANDES FORMIGHIERI, CNPAE; MARCOS F. BASSO; JOSE RENATO BOUCAS FARIAS, CNPSO; BEATRIZ MARTI EMYGDIO, CPACT; ANA CLAUDIA BARNECHE DE OLIVEIRA, CPACT; ANGELA DINIZ CAMPOS, CPACT; ALUÍZIO BORÉM, UFV; FRANK G. HARMON, University of California-Berkley; LILIANE MARCIA MERTZ HENNING, CNPSO; ALEXANDRE LIMA NEPOMUCENO, CNPSO.
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