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http://www.alice.cnptia.embrapa.br/alice/handle/doc/1082755Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | HE, Y. | eng |
| dc.contributor.author | MATHIEU, J. | eng |
| dc.contributor.author | YANG, Y. | eng |
| dc.contributor.author | YU, P. | eng |
| dc.contributor.author | SILVA, M. L. B. da | eng |
| dc.contributor.author | ALVAREZ, P. J. J. | eng |
| dc.date.accessioned | 2019-06-19T01:14:04Z | - |
| dc.date.available | 2019-06-19T01:14:04Z | - |
| dc.date.created | 2017-12-18 | |
| dc.date.issued | 2017 | |
| dc.identifier.citation | Environmental Science & Technology, v. 4, n. 11, p. 494-499, 2017. | eng |
| dc.identifier.uri | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1082755 | - |
| dc.description | ABSTRACT: 1,4-Dioxane (dioxane) is a groundwater contaminant of emerging concern for which bioremediation may be a promising strategy. Several bacterial strains can metabolize dioxane or degrade it cometabolically. However, the molecular basis of dioxane biodegradation is only partially understood, and the gene coding for dioxane/ tetrahydrofuran (THF) monooxygenase in Pseudonocardia dioxanivorans CB1190 is the only well-characterized catabolic gene. Here, we identify a novel group-6 propane monooxygenase gene cluster (prmABCD) in Mycobacterium dioxanotrophicus PH-06, which is a bacterium with superior dioxane degradation kinetics compared with CB1190. Whole genome sequencing of PH-06 revealed the existence of a single soluble di-iron monooxygenase (SDIMO). RNA sequencing and reverse transcription quantitative PCR (RT-qPCR) subsequently confirmed that all four components of this gene cluster are upregulated when PH-06 is grown on dioxane compared with growth on acetate or glucose as negative controls. This first characterization of a group-6 SDIMO associated with dioxane biodegradation suggests that dioxane-degrading genes may be more diverse than previously appreciated. A primer/probe set designed to target the large hydroxylase subunit of this gene cluster exhibited high selectivity (no false positives) and high sensitivity (detection limit = 3000−4000 gene copies/mL culture), which may be useful to help assess the presence of dioxane degraders at contaminated sites and minimize false negatives. | eng |
| dc.language.iso | eng | eng |
| dc.rights | openAccess | eng |
| dc.subject | Microbactéria | eng |
| dc.subject | Biodegradação de dioxano | eng |
| dc.title | Dioxane biodegradation by mycobacterium dioxanotrophicus pH-06 is associated with a group-6 soluble di-iron monooxygenase. | eng |
| dc.type | Artigo de periódico | eng |
| dc.date.updated | 2019-06-19T01:14:04Z | |
| riaa.ainfo.id | 1082755 | eng |
| riaa.ainfo.lastupdate | 2019-06-18 | |
| dc.identifier.doi | 10.1021/acs.estlett.7b00456 | eng |
| dc.contributor.institution | YA HE, Rice University Houston - Texas; JACQUES MATHIEU, Rice University Houston - Texas; YU YANG, Rice University Houston - Texas; PINGFENG YU, Rice University Houston - Texas; MARCIO LUIS BUSI DA SILVA, CNPSA; PEDRO J. J. ALVAREZ, Rice University Houston - Texas. | eng |
| Appears in Collections: | Artigo em periódico indexado (CNPSA)  | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| marciobusi.pdf | 1.08 MB | Adobe PDF |  View/Open |
