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Title: | Characterization of Staphylococcus spp. strains in milk from buffaloes with mastitis in Brazil: the need to identify to species level to avoid misidentification. |
Authors: | COIMBRA E SOUZA, V.![]() ![]() BRITO, M. A. V. P. e ![]() ![]() CHAMON, R. C. ![]() ![]() LAPORT, M. S. ![]() ![]() GIAMBIAGI DE MARVAL, M. ![]() ![]() |
Affiliation: | UFRRJ; MARIA APARECIDA V PAIVA E BRITO, CNPGL; UFRRJ; UFRRJ; UFRRJ. |
Date Issued: | 2017 |
Citation: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 69, n. 6, p. 1669-1675, 2017. |
Description: | ABSTRACT - Mastitis is an inflammation of the mammary gland that affects dairy cattle worldwide causing economic losses. Coagulase-negative staphylococci (CNS) are the predominant cause of this type of infection. We have recently showed that coagulase-positive staphylococci could be misidentified. So, the aim of this study was to characterize the Staphylococcus spp. strains initially classified as coagulase-negative Staphylococci, isolated from buffalo with subclinical mastitis. Milk of buffaloes with mastitis in herds was collected and 9 strains were identified as CNS by phenotypic tests. Molecular methodologies latter identified the strains as coagulase-negative Staphylococcus chromogenes (5), coagulase-positive Staphylococcus hyicus (2) and coagulase-positive Staphylococcus aureus (2). Our results strongly support the need to identify the isolates to a species level in order to avoid misidentification and to be aware of the classification using the coagulase test alone. RESUMO - A mastite é uma inflamação da glândula mamária que afeta o gado leiteiro em todo o mundo, causando perdas econômicas. Staphylococcus coagulase-negativa (SCN) são a causa predominante desse tipo de infecção. Mostrou-se recentemente que Staphylococcus coagulase-positiva podem ser identificados erroneamente. Assim, o objetivo deste estudo foi caracterizar cepas de Staphylococcus spp. inicialmente classificados como Staphylococcus coagulase-negativa, isolados de búfalas com mastite subclínica. O leite de búfalas com mastite foi coletado, e nove cepas foram identificadas como SCN por testes fenotípicos. Metodologias moleculares identificaram as cepas como Staphylococcus chromogenes coagulase-negativa (5) Staphylococcus hyicus coagulase-positiva (2) e Staphylococcus aureus coagulase-positiva (2). Os resultados reforçam a necessidade de identificar as cepas em termos de espécie, a fim de se evitarem erros de identificação e estar atento à classificação utilizando o teste de coagulase sozinho. |
NAL Thesaurus: | Staphylococcus mastitis milk |
Keywords: | Buffalo Coagulase test |
DOI: | https://doi.org/10.1590/1678-4162-9351 |
Type of Material: | Artigo de periódico |
Access: | openAccess |
Appears in Collections: | Artigo em periódico indexado (CNPGL)![]() ![]() |
Files in This Item:
File | Description | Size | Format | |
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Artigo-Characterization-of-Staphylococcus.pdf | 278.89 kB | Adobe PDF | ![]() View/Open |