Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1087004
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dc.contributor.authorSANTOS, S. N.
dc.contributor.authorMELO, I. S. de
dc.contributor.otherSUIKINAI NOBRE SANTOS, FAPESP; ITAMAR SOARES DE MELO, CNPMA.
dc.date.accessioned2018-02-02T18:33:46Z-
dc.date.available2018-02-02T18:33:46Z-
dc.date.created2018-02-02
dc.date.issued2016
dc.identifier.other16009
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1087004-
dc.descriptionInvestigating bioactive components from microbial metabolites can provide potential source for drug discovery. There are a number of traditional antitumor compounds screening assays already described. In this study, we have developed an efficient method to search for antitumor compounds using the Oomycete Pythium aphanidermatum as model on agar plates for high throughtput screening. Antioomycete compounds showed cytotoxic effects in human cancer cells lines due to the possible similarity between the lipid cell membrane of the Pythium species and cancerous cells, suggesting the compounds act through similar mechanisms of inhibition. In this assay, i t is possible to evaluate hundreds of samples, with results in few hours. The Pythium assay was shown to be sensitive for several chemical substances and allowed the detection and isolation of antitumor compounds from microorganisms. This bioassay was shown to be sensitive, rapid, simple and reliable for antitumor activity. It takes just few hours to evaluate the properties of the extracts.
dc.description.uribitstream/item/172110/1/2017AP61.pdfpt_BR
dc.languageen
dc.language.isoengeng
dc.publisherNatural Products Chemistry & Research, v. 4, n. 6, 2016.
dc.relation.ispartofEmbrapa Meio Ambiente - Artigo em periódico indexado (ALICE)
dc.subjectAntitumoral screening
dc.subjectAntifungal assay.
dc.titleA rapid primary screening method for antitumor using the oomycete Pythium aphanidermatum.
dc.typeArtigo em periódico indexado (ALICE)
dc.date.updated2018-03-09T11:11:11Zpt_BR
dc.subject.thesagroPythium Aphanidermatum.pt_BR
dc.subject.nalthesaurusAntineoplastic agents
dc.subject.nalthesaurusScreening
dc.subject.nalthesaurussecondary metabolites.pt_BR
dc.ainfo.id1087004
dc.ainfo.lastupdate2018-03-09 -03:00:00
dc.identifier.doi10.4172/2329-6836.1000241
Appears in Collections:Artigo em periódico indexado (CNPMA)

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