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dc.contributor.authorFERRÃO, L. F. V.
dc.contributor.authorCAIXETA, E. T.
dc.contributor.authorSOUZA, F. de F.
dc.contributor.authorZAMBOLIM, E. M.
dc.contributor.authorCRUZ, C. D.
dc.contributor.authorZABOLIM, L.
dc.contributor.authorSAKIYAMA, N. S.
dc.date.accessioned2018-02-28T00:53:27Z-
dc.date.available2018-02-28T00:53:27Z-
dc.date.created2018-02-27
dc.date.issued2013
dc.identifier.citationPlant Systematics and Evolution, Springer Verlag, New York, v. 299, p. 225-238, 2013.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1088232-
dc.descriptionThe genetic variability characterization of the accessions of the germplasm collection, using molecular markers, is being applied as a complementary strategy to the traditional approaches to redefine the plant genetic resources. In this study, we compared the informativeness and efficiency of the molecular markers RAPD, AFLP and SSR in the analysis of 94 accessions of Coffea canephora germplasm held by the breeding program of the Brazilian Agricultural Research Corporation (Embrapa), Rondonia State, Brazil. For this, we considered the marker?s discriminatory power and level of polymorphism detected and also the genetic relationships and clustering (dendrogram) analysis. The RAPD marker yielded low-quality data and problems in the discrimination of some accessions, being less recommended for genetic studies of C. canephora . The SSRs had a higher level of information content and yielded high-quality data, while AFLP was the most efficient marker system because of the simultaneous detection of abundant polymorphism markers per few reactions. Our results indicate that AFLP and SSR, allies to the intrinsic characteristics of each technique, are the most suitable molecular markers for genetic studies of C. canephora .However, the choice of AFLP or SSR in the species characterization should be made in agreement with some characteristics that are discussed in this work.
dc.language.isoporpt_BR
dc.rightsopenAccesspt_BR
dc.subjectAFLP
dc.subjectSSR
dc.subjectRAPD
dc.subjectRobusta coffee
dc.subjectMelhoramento genético
dc.subjectCoffeept_BR
dc.titleComparative study of different molecular markers for classifying and establishing genetic relationships in Coffea Canephora.
dc.typeArtigo de periódico
dc.date.updated2018-02-28T00:53:27Zpt_BR
dc.subject.thesagroCafé
dc.subject.nalthesaurusgenetic relationshipspt_BR
riaa.ainfo.id1088232
riaa.ainfo.lastupdate2018-02-27
dc.contributor.institutionLUÍS FELIPE V. FERRÃO, Instituto de Biotecnologia Aplicada á Agropecuária(BIOAGRO) Laboratório BIOCAFÉ, Universidade Federal de Viçosa,MG; EVELINE TEIXEIRA CAIXETA, SAPC; FLAVIO DE FRANCA SOUZA, CPATSA; EUNIZE M.ZAMBOLIM, Instituto de Biotecnologia Aplicada áAgropecuária(BIOAGRO), Laboratório BIOCAFÉ, Universidade Federal de Viçosa, MG; COSME D.CRUZ, Departamento de Biologia Geral, Universidade Federal de Viçosa, MG; LAÉRCIO ZAMBOLIM, Departamento de Fitopatologia, Univerdade Federal de Viçosa, MG; NEY S. SAKIYAMA, Departamento de Fitotecnia, Universidade Federal de Viçosa, MG.
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