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dc.contributor.authorMOREIRA, R. F.
dc.contributor.authorMATOS, M. N. C.
dc.contributor.authorALVES FILHO, J. G.
dc.contributor.authorVALLE, R. V. do
dc.contributor.authorELOY, A. M. X.
dc.contributor.authorPINTO, T. M. F.
dc.contributor.authorMACHADO, S. P.
dc.contributor.authorCOSTA, C. R. R.
dc.contributor.authorLIMA FILHO, J. L. de
dc.contributor.authorLIMA, J. P. M. S.
dc.contributor.authorCUNHA, R. M. S. da
dc.date.accessioned2018-04-20T01:08:20Z-
dc.date.available2018-04-20T01:08:20Z-
dc.date.created2018-04-12
dc.date.issued2018
dc.identifier.citationAnimal Reproduction, Belo Horizonte, v. 15, n. 1, p. 84-92, jan./mar. 2018.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1090515-
dc.descriptionAbstract: This study aimed to develop protocols for the extraction of sperm proteins from Moxotó goats (Capra hircus) and to compare the resulting proteomic maps. The sperm proteins were isolated using an extraction buffer containing 7 M urea and 2 M thiourea, 20 mM DTT, and one of the following detergents: 1% or 4% CHAPS; 1% or 4% SDS; 1% or 4% Triton X-100; or a combination of CHAPS and SDS. The 1-DE and 2-DE profiles of the isolated proteins revealed that the various isolation methods were efficient. Qualitative and quantitative differences in the 1-DE and 2-DE profiles were observed. 2-DE maps indicated that the amount and diversity of proteins visualized depended on the detergent that was used. Furthermore, this work revealed that the combination of detergents increased the resolution of some spots and retained the characteristics of the individual detergents, depending on their concentrations.
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectIsolation methods
dc.subjectProteomic profiles
dc.subjectProteomic analysis
dc.subjectRaça Moxotó
dc.subjectAnálise proteômica
dc.titleDiversity of ejaculated sperm proteins in Moxotó bucks (Capra hircus) evaluated by multiple extraction methods.
dc.typeArtigo de periódico
dc.date.updated2019-04-08T11:11:11Zpt_BR
dc.subject.thesagroCaprino
dc.subject.thesagroDetergente
dc.subject.nalthesaurusGoats
dc.subject.nalthesaurusSurfactants
dc.subject.nalthesaurusSpermatozoa
dc.subject.nalthesaurusdetergents
dc.subject.nalthesaurusproteomics
riaa.ainfo.id1090515
riaa.ainfo.lastupdate2019-04-08 -03:00:00
dc.contributor.institutionRaulzito Fernandes Moreira, Pós-graduação - Universidade Federal do Ceará (UFC) - Sobral, CE, Brasil.
dc.contributor.institutionMaria Nágila Carneiro Matoseng
dc.contributor.institutionJoão Garcia Alves Filhoeng
dc.contributor.institutionRoberta Vianna do Valleeng
dc.contributor.institutionANGELA MARIA XAVIER ELOY, CNPCeng
dc.contributor.institutionTatiana Maria Farias Pintoeng
dc.contributor.institutionSaris Pinto Machado Junioreng
dc.contributor.institutionCíntia Renata Rocha Costaeng
dc.contributor.institutionJosé Luiz de Lima Filhoeng
dc.contributor.institutionJoão Paulo Matos Santos Limaeng
dc.contributor.institutionRodrigo Maranguape Silva da Cunha.eng
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