Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1092380
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dc.contributor.authorBOSCO, D. D.
dc.contributor.authorSINSKI, I.
dc.contributor.authorRITSCHEL, P. S.
dc.contributor.authorCAMARGO, U. A.
dc.contributor.authorFAJARDO, T. V. M.
dc.contributor.authorHARAKAVA, R.
dc.contributor.authorQUECINI, V.
dc.contributor.otherDANIELA DAL BOSCO, CNPUV; IRACI SINSKI, CNPUV; PATRICIA SILVA RITSCHEL, CNPUV; Umberto A. Camargo; THOR VINICIUS MARTINS FAJARDO, CNPUV; Ricardo Harakava; VERA MARIA QUECINI, CNPUV.
dc.date.accessioned2018-06-09T01:22:40Z-
dc.date.available2018-06-09T01:22:40Z-
dc.date.created2018-06-08
dc.date.issued2018
dc.identifier.other17727
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1092380-
dc.descriptionIncreased tolerance to pathogens is an important goal in conventional and biotechnology-assisted grapevine breeding programs worldwide. Fungal and viral pathogens cause direct losses in berry production, but also affect the quality of the final products. Precision breeding strategies allow the introduction of resistance characters in elite cultivars, although the factors determining the plant?s overall performance are not fully characterized. Grapevine plants expressing defense proteins, from fungal or plant origins, or of the coat protein gene of grapevine leafroll-associated virus 3 (GLRaV-3) were generated by Agrobacterium -mediated transformation of somatic embryos and shoot apical meristems. The responses of the transformed lines to pathogen challenges were investigated by biochemical, phytopathological and molecular methods. The expression of a Metarhizium anisopliae chitinase gene delayed pathogenesis and disease progression against the necrotrophic pathogen Botrytis cinerea. Modified lines expressing a Solanum nigrum osmotin-like protein also exhibited slower disease progression, but to a smaller extent. Grapevine lines carrying two hairpin- inducing constructs had lower GLRaV-3 titers when challenged by grafting, although disease symptoms and viral multiplication were detected. The levels of global genome methylation were determined for the genetically engineered lines, and correlation analyses demonstrated the association between higher levels of methylated DNA and larger portions of virus-derived sequences. Resistance expression was also negatively correlated with the contents of introduced viral sequences and genome methylation, indicating that the effectiveness of resistance strategies employing sequences of viral origin is subject to epigenetic regulation in grapevine. Keywords Chitinase, Epigenetics, Fungus, Grapevine leafroll-associated virus 3, Pathogenesis related protein 5, Vitis
dc.description.uribitstream/item/178409/1/s11248-018-0082-1-Artigo-v-publicada-Metilacao-DNA-Transgenic-Research-06-06-18.pdfpt_BR
dc.languagept_BR
dc.language.isoporpt_BR
dc.publisherTransgenic Research, p. 1-18, 2018. [Online]
dc.relation.ispartofEmbrapa Uva e Vinho - Artigo em periódico indexado (ALICE)
dc.rightsopenAccesspt_BR
dc.subjectPathogenesis related protein 5
dc.subjectFungus
dc.titleExpression of disease resistance in genetically modified grapevines correlates with the contents of viral sequences in the T-DNA and global genome methylation.
dc.typeArtigo em periódico indexado (ALICE)
dc.date.updated2019-04-29T11:11:11Zpt_BR
dc.subject.nalthesaurusChitinase
dc.subject.nalthesaurusEpigenetics
dc.subject.nalthesaurusGrapevine leafroll-associated virus 3
dc.subject.nalthesaurusVitis
dc.ainfo.id1092380
dc.ainfo.lastupdate2019-04-29 -03:00:00
dc.identifier.doihttps://doi.org/10.1007/s11248-018-0082-1
Appears in Collections:Artigo em periódico indexado (CNPUV)

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