Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1094508
Research center of Embrapa/Collection: Embrapa Cerrados - Artigo em periódico indexado (ALICE)
Date Issued: 2018
Type of Material: Artigo em periódico indexado (ALICE)
Authors: SOUZA, L. M. de
SANTOS, L. H. B. dos
ROSA, J. R. B. F.
SILVA, C. C. da
MANTELLO, C. C.
CONSON, A. R. O.
SCALOPPI JUNIOR, E. J.
FIALHO, J. de F.
MORAES, M. L. T. de
GONÇALVES, P. de S.
MARGARIDO, G. R. A.
GARCIA, A. A. F.
LE GUEN, V.
SOUZA, A. P. de
Additional Information: LIVIA M. DE SOUZA, UNICAMP; LUCIANO H. B. DOS SANTOS, UNICAMP; JOÃO R. B. F. ROSA, USP/ESALQ; CARLA C. DA SILVA, UNICAMP; CAMILA C. MANTELLO, UNICAMP; ANDRE R. O. CONSON, UNICAMP; ERIVALDO J. SCALOPPI JUNIOR, IAC; JOSEFINO DE FREITAS FIALHO, CPAC; MARIO LUIZ T. DE MORAES, UNESP; PAULO DE S. GONÇALVES, IAC; GABRIEL R. A. MARGARIDO, USP/ESALQ; ANTONIO A. F. GARCIA, USP/ESALQ; VINCENT LE GUEN, CIRAD; ANETE P. DE SOUZA, UNICAMP.
Title: Linkage disequilibrium and population structure in wild and cultivated populations of rubber tree (Hevea brasiliensis).
Publisher: Frontiers in Plant Science, v. 9, article 815, 03 July 2018.
Language: en
Description: Abstract: Among rubber tree species, which belong to the Hevea genus of the Euphorbiaceae family, Hevea brasiliensis (Willd. ex Adr.de Juss.) Muell. Arg. is the main commercial source of natural rubber production worldwide. Knowledge of the population structure and linkage disequilibrium (LD) of this species is essential for the efficient organization and exploitation of genetic resources. Here, we obtained single-nucleotide polymorphisms (SNPs) using a genotyping-by-sequencing (GBS) approach and then employed the SNPs for the following objectives: (i) to identify the positions of SNPs on a genetic map of a segregating mapping population, (ii) to evaluate the population structure of a germplasm collection, and (iii) to detect patterns of LD decay among chromosomes for future genetic association studies in rubber tree. A total of 626 genotypes, including both germplasm accessions (368) and individuals from a genetic mapping population (254), were genotyped. A total of 77,660 and 21,283 SNPs were detected by GBS in the germplasm and mapping populations, respectively. The mapping population, which was previously mapped, was constructed with 1,062 markers, among which only 576 SNPs came from GBS, reducing the average interval between two adjacent markers to 4.4 cM. SNPs from GBS genotyping were used for the analysis of genetic structure and LD estimation in the germplasm accessions. Two groups, which largely corresponded to the cultivated and wild populations, were detected using STRUCTURE and via principal coordinate analysis. LD analysis, also using the mapped SNPs, revealed that non-random associations varied along chromosomes, with regions of high LD interspersed with regions of low LD. Considering the length of the genetic map (4,693 cM) and the mean LD (0.49 for cultivated and 0.02 for wild populations), a large number of evenly spaced SNPs would be needed to perform genome-wide association studies in rubber tree, and the wilder the genotypes used, the more difficult the mapping saturation.
Thesagro: Seringueira
Hevea Brasiliensis
Comportamento de Variedade
População de Planta
Variação Genética
Data Created: 2018-08-24
Appears in Collections:Artigo em periódico indexado (CPAC)

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