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dc.contributor.authorPFEIFER, L. F. M.
dc.contributor.authorANDRADE, J. de S.
dc.contributor.authorMOREIRA, E. M.
dc.contributor.authorSILVA, R. R. da
dc.contributor.authorNEVES, P. M. A.
dc.contributor.authorSILVA, G. M. da S.
dc.contributor.authorLEMOS, I. C.
dc.contributor.authorSCHNEIDER, A.
dc.date.accessioned2018-09-19T00:37:45Z-
dc.date.available2018-09-19T00:37:45Z-
dc.date.created2018-09-18
dc.date.issued2018
dc.identifier.citationAnimal Reproduction Science, v. 197, p. 268-277, 2018.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1095927-
dc.descriptionThe aim of this study was to evaluate the uterine health and fertility of postpartum beef cows subjected to timed AI (TAI) protocols at different days from calving. Lactating Nelore cows (Bos indicus; n = 244) were used in this study. The TAI protocols were initiated between 20 and 60 days postpartum (DPP). Cows were subjected to an estradiol-progesterone based TAI protocol. The cows were divided into three groups according to the days postpartum at the time the hormonal treatment was initiated: 1) Early (n = 64), cows between 20 and 30 DPP; 2) Middle (n = 115), cows between 31 and 45 DPP; and 3) Late (n = 65), cows between 46 and 60 DPP. At Day 0 of the protocol, endometrial cytobrush samples were collected. Slides for polymorphonuclear (PMN) cell counting were prepared and cytobrush was used for RNA extraction and analysis of relative abundance of il1, il6, il8, and tnf mRNA. Cows from the Early group had less (P < 0.05) pregnancies per AI (P/AI) than cows from Middle and Late groups; 29.7% (19/64), 45.2% (52/115), and 52.31% (34/65), respectively. Accordingly, the Early group had a greater (P < 0.05) proportion of PMN cells in the uterus than Middle and Late groups; 9.0%, 3.8%, and 2.2%, respectively. Relative abundances of il1 and il8 mRNA were greater (P < 0.05) in the Early group than Middle and Late groups. These results indicate that beef cows subjected to TAI protocols early postpartum (< 30 DPP) are less likely to become pregnant, which is associated with increased inflammation and relative abundance of mRNA for the proinflammatory cytokines il1 and il8.
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectCytobrush
dc.subjectPMN cell
dc.subjectCélulas polimorfonucleares
dc.subjectFertilidade uterina
dc.subjectBovino de corte
dc.titleUterine inflammation and fertility of beef cows subjected to timed AI at different days postpartum.
dc.typeArtigo de periódico
dc.date.updated2018-09-19T00:37:45Zpt_BR
dc.subject.thesagroFertilidade Animal
dc.subject.nalthesaurusCalving
dc.subject.nalthesaurusCytokines
riaa.ainfo.id1095927
riaa.ainfo.lastupdate2018-09-18
dc.identifier.doihttps://doi.org/10.1016/j.anireprosci.2018.08.039
dc.contributor.institutionLUIZ FRANCISCO MACHADO PFEIFER, CPAF-RO; Jéssica de Souza Andrade, Bionorte, Programa de Pós-Graduação em Biodiversidade e Biotecnologia; Elizângela Mírian Moreira; RENATA REIS DA SILVA, CPAF-RO; Paulo Marcos Araújo Neves, UNIR; George Moreira da Silva, UNIR; Izabela Cristina Lemos, UNIR; Augusto Schneider, UFPel.
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