Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1100838
Research center of Embrapa/Collection: Embrapa Suínos e Aves - Artigo em periódico indexado (ALICE)
Date Issued: 2018
Type of Material: Artigo em periódico indexado (ALICE)
Authors: SOUZA, R. C.
CANTAO, M. E.
NOGUEIRA, M. A.
VASCONCELOS, A. T. R.
HUNGRIA, M.
Additional Information: RENATA CAROLINI SOUZA, CNPq; MAURICIO EGIDIO CANTAO, CNPSA; MARCO ANTONIO NOGUEIRA, CNPSO; ANA TEREZA RIBEIRO VASCONCELOS, CNPq; MARIANGELA HUNGRIA DA CUNHA, CNPSO.
Title: Outstanding impact of soil tillage on the abundance of soil hydrolases revealed by a metagenomic approach.
Publisher: Brazilian Journal of Microbiology, v. 49, p. 723-730, 2018.
Language: en
Keywords: Metagenome
Description: Abstract: The soil represents the main source of novel biocatalysts and biomolecules of industrial relevance. We searched for hydrolases in silico in four shotgun metagenomes (4,079,223 sequences) obtained in a 13-year field trial carried out in southern Brazil, under the no-tillage (NT), or conventional tillage (CT) managements, with crop succession (CS, soybean/wheat), or crop rotation (CR, soybean/maize/wheat/lupine/oat). We identified 42,631 hydrolases belonging to five classes by comparing with the KEGG database, and 44,928 sequences by comparing with the NCBI-NR database. The abundance followed the order: lipases > laccases > cellulases > proteases > amylases > pectinases. Statistically significant differences were attributed to the tillage system, with the NT showing about five times more hydrolases than the CT system. The outstanding differences can be attributed to the management of crop residues, left on the soil surface in the NT, and mechanically broken and incorporated into the soil in the CT. Differences between the CS and the CR were slighter, 10% higher for the CS, but not statistically different. Most of the sequences belonged to fungi (Verticillium, and Colletotrichum for lipases and laccases, and Aspergillus for proteases), and to the archaea Sulfolobus acidocaldarius for amylases. Our results indicate that agricultural soils under conservative managements may represent a hotspotfor bioprospection of hydrolases.
Thesagro: Manejo do Solo
Plantio Direto
Microbiologia do Solo
NAL Thesaurus: Soil management
Soil enzymes
No-tillage
Metagenomics
Microbiome
Data Created: 2018-12-06
Appears in Collections:Artigo em periódico indexado (CNPSA)

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