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| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.contributor.author | OTERO, R. | |
| dc.contributor.author | HERNÁNDEZ, D. | |
| dc.contributor.author | CAMARGO, L. S. de A. | |
| dc.date.accessioned | 2018-12-26T23:42:43Z | - |
| dc.date.available | 2018-12-26T23:42:43Z | - |
| dc.date.created | 2018-12-26 | |
| dc.date.issued | 2018 | |
| dc.identifier.citation | Indian Journal of Science and Technology, v. 11, n. 25, 2018. | |
| dc.identifier.uri | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1102530 | - |
| dc.description | Abstract Objective: To evaluate the effect of treatment with trichostatin-A (TSA) on the production of bovine embryos, expressing the gene of the green fluorescent protein (GFP) generated by SCNT. Materials: 164 oocytes were distributed in three treatments, NT-GFP: newly reconstructed zygotes with genetically modified cells and not subject to TSA. NT-Trico-GFP: newly reconstructed zygotes with genetically modified cells and subjected to TSA. PART: Zygotes generated by parthenogenetic activation, used as a control for the process of oocyte activation and culture of embryos. The rates of cleavage, blastocysts, and embryos that expressed GFP were assessed by contingency tables and chi-square tests. Results: The percentage of cleavage in the zygotes in the NT-GFP treatment was greater but did not vary significantly from the NT-Trico-GFP treatment. However, this last treatment had a higher percentage of blastocyst formation (p = 0.077). The percentage of blastocysts from cleaved zygotes, the produced embryos were significantly higher (p < 0.05) for the NT-Trico-GFP treatment than for the NT-GFP. In both treatments, all the blastocysts generated expressed the GFP protein. Conclusions:TSA improves the embryonic development of clones of genetically modified cattle that express GFP. | |
| dc.language.iso | eng | eng |
| dc.rights | openAccess | eng |
| dc.subject | Embryonic Development | |
| dc.subject | Epigenetic Modification | |
| dc.subject | Nuclear Transfer | |
| dc.title | Effect of Trichostatin-A on embryos of bovine clones modified genetically with GFP. | |
| dc.type | Artigo de periódico | |
| dc.date.updated | 2019-01-04T11:11:11Z | pt_BR |
| dc.format.extent2 | 9 p. | |
| riaa.ainfo.id | 1102530 | |
| riaa.ainfo.lastupdate | 2019-01-04 -02:00:00 | |
| dc.identifier.doi | 10.17485/ijst%2F2018%2Fv11i25%2F128251 | |
| dc.contributor.institution | LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. | |
| Aparece en las colecciones: | Artigo em periódico indexado (CNPGL)  | |
Ficheros en este ítem:
| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| ArtigoIndianJSciandTechCamargoEffect.pdf | 383.61 kB | Adobe PDF |  Visualizar/Abrir |
