Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1103147
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dc.contributor.authorMATTOS, V. da S.
dc.contributor.authorMULET, K.
dc.contributor.authorCARES, J. E.
dc.contributor.authorGOMES, C. B.
dc.contributor.authorFERNANDEZ, D.
dc.contributor.authorSA, M. F. G. dept_BR
dc.contributor.authorCARNEIRO, R. M. D. G.pt_BR
dc.contributor.authorCASTAGNONE-SERENO, P.pt_BR
dc.date.accessioned2019-01-07T23:50:25Z-
dc.date.available2019-01-07T23:50:25Z-
dc.date.created2019-01-07
dc.date.issued2019
dc.identifier.citationPlant Disease, v. 103, p. 83-88, Jan. 2019.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1103147-
dc.descriptionRoot-knot nematodes (RKN) cause important production losses of rice (Oryza sativa L.) in the world. Together with Meloidogyne graminicola Golden and Birchfield 1965, M. oryzae Maas, Sanders and Dede, 1978 and M. salasi López, 1984 have been causing damages in irrigated rice fields in Central and South America. In addition, six other RKN species may occur in rice fields in other regions of the world. Correct identification of Meloidogyne spp. is difficult but essential for the management of rice RKNs. The objective of this study was to develop some species-specific molecular markers for the diagnosis of South American RKN rice-related species. Isozyme phenotypes indicated the occurrence of some RKN species in the Brazilian samples, namely M. graminicola, M. oryzae, M. javanica, and two cryptic species designated as Meloidogyne sp. 2 and Meloidogyne sp. 3. Random amplified polymorphic DNA (RAPD) analysis of 16 isolates revealed interspecific genetic polymorphism between Meloidogyne spp., but isolates belonging to the same species (i.e., sharing the same esterase phenotype) always clustered together, whatever the species considered. Specific SCAR markers of 230, 120, and 160 bp were developed for M. graminicola, M. oryzae, and M. salasi, respectively. These SCAR markers may be potential molecular tools for application in routine diagnostic procedures subject to their validation with other rice RKN field populations in the world.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectNematóide das galhaspt_BR
dc.titleDevelopment of Diagnostic SCAR Markers for Meloidogyne graminicola, M. Oryzae, and M. salasi associated with irrigated rice fields in Americas.
dc.typeArtigo de periódico
dc.date.updated2019-12-18T11:11:11Zpt_BR
dc.subject.thesagroOryza Sativa
dc.subject.thesagroMarcador Molecularpt_BR
dc.subject.thesagroNematóidept_BR
riaa.ainfo.id1103147
riaa.ainfo.lastupdate2019-12-18 -02:00:00
dc.identifier.doihttps://doi.org/10.1094/PDIS-12-17-2015-REpt_BR
dc.contributor.institutionVANESSA DA SILVA MATTOS, UNB
dc.contributor.institutionKARINE MULET, CNRSpt_BR
dc.contributor.institutionJUVENIL ENRIQUE CARES, UNBpt_BR
dc.contributor.institutionCESAR BAUER GOMES, CPACTpt_BR
dc.contributor.institutionDIANA FERNANDEZ, IRDpt_BR
dc.contributor.institutionMARIA FATIMA GROSSI DE SA, Cenargenpt_BR
dc.contributor.institutionREGINA MARIA DECHECHI G CARNEIRO, Cenargenpt_BR
dc.contributor.institutionPHILIPPE CASTAGNONE-SERENO, INRA.pt_BR
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