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dc.contributor.authorMALABARBA, J.
dc.contributor.authorBUFFON, V.
dc.contributor.authorMARIATH, J. E. A.
dc.contributor.authorMARASHIN, F. S.
dc.contributor.authorMARGIS-PINHEIRO, M.
dc.contributor.authorPASQUALI, G.
dc.contributor.authorREVERS, L. F.
dc.date.accessioned2019-01-07T23:54:59Z-
dc.date.available2019-01-07T23:54:59Z-
dc.date.created2019-01-07
dc.date.issued2018
dc.identifier.citationPlant Science, v. 269, p. 126-135, 2018.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1103200-
dc.descriptionSeedlessness in grapes is a desirable trait, especially for in natura consumption. Previously, we showed that VviAGL11 is the main responsible gene for seed morphogenesis in grapevine. Here we tested the function of this gene in grapevine with the use of plant plasmids. VviAGL11 was cloned into silencing and overexpression versions of p28iIR plasmid. Reproductive grapevine bunches from di ff erent seeded and seedless cultivars were separately treated with VviAGL11 -harboring plasmids, along with controls. Plasmids were detected in leaves after a month of treatment, and berries, leaves, stems and seeds were analyzed for ectopic gene expression by RT- qPCR after 90 days of plasmid injection. Fruits from the seedless ? Linda ? treated with the VviAGL11-over- expression plasmid showed high expression levels of VviAGL11 and exhibited small seeds that were not found in the untreated control samples. Mature grapes from seeded ? Italia ? and ? Ruby ? bunches treated with the VviAGL11-silencing plasmid showed decreased VviAGL11 expression, reduced number of seeds and increased number of seed traces. The present study con fi rms that VviAGL11 is a key master regulator of seed morpho- genesis in grapevine and corroborates with the applicability of plant plasmids as promising biotechnological tools to functionally test genes in perennial plants in a rapid and confident way. Keywords: Plant plasmid Grapevine Seed Silencing Overexpression VviAGL11
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectPlant plasmid
dc.subjectGrapevine
dc.subjectSeed
dc.subjectSilencing
dc.subjectOverexpression
dc.subjectVviAGL11
dc.titleManipulation of VviAGL11 expression changes the seed content in grapevine (Vitis vinifera L.).
dc.typeArtigo de periódico
dc.date.updated2019-04-27T11:11:11Zpt_BR
riaa.ainfo.id1103200
riaa.ainfo.lastupdate2019-04-27 -03:00:00
dc.contributor.institutionJaiana Malabarba, Graduate Program in Cell and Molecular Biology, Center for Biotechnology, Federal University of Rio Grande do Sul, Porto Alegre, RS, 91501-970, BraZIL; VANESSA BUFFON, CNPUV; Jorge E.A. Mariath, raduate Program in Botany, Botany Department, Institute for Bioscience, Federal University of Rio Grande do Sul, Porto Alegre, RS, 91501-970, Brazil; Felipe S. Maraschin, raduate Program in Botany, Botany Department, Institute for Bioscience, Federal University of Rio Grande do Sul, Porto Alegre, RS, 91501-970, Brazil; Márcia Margis-Pinheiro, Graduate Program in Cell and Molecular Biology, Center for Biotechnology, Federal University of Rio Grande do Sul, Porto Alegre, RS, 91501-970, Brazil; Giancarlo Pasquali, Graduate Program in Cell and Molecular Biology, Center for Biotechnology, Federal University of Rio Grande do Sul, Porto Alegre, RS, 91501-970, Brazil; LUIS FERNANDO REVERS, CNPUV.
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