Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1104582
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dc.contributor.authorALVES-FREITAS, D. M. T.
dc.contributor.authorPINHEIRO-LIMA, B.
dc.contributor.authorFARIA, J. C.
dc.contributor.authorLACORTE, C.
dc.contributor.authorRIBEIRO, S. G.
dc.contributor.authorMELO, F. L.
dc.date.accessioned2019-01-22T00:09:37Z-
dc.date.available2019-01-22T00:09:37Z-
dc.date.created2019-01-21
dc.date.issued2019
dc.identifier.citationViruses, v. 11, n. 1, Jan. 2019.
dc.identifier.issn1999-4915
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1104582-
dc.descriptionUsing double-strand RNA (dsRNA) high-throughput sequencing, we identified five RNA viruses in a bean golden mosaic virus (BGMV)-resistant common bean transgenic line with symptoms of viral infection. Four of the identified viruses had already been described as infecting common bean (cowpea mild mottle virus, bean rugose mosaic virus, Phaseolus vulgaris alphaendornavirus 1, and Phaseolus vulgaris alphaendornavirus 2) and one is a putative new plant rhabdovirus (genus Cytorhabdovirus), tentatively named bean-associated cytorhabdovirus (BaCV). The BaCV genome presented all five open reading frames (ORFs) found in most rhabdoviruses: nucleoprotein (N) (ORF1) (451 amino acids, aa), phosphoprotein (P) (ORF2) (445 aa), matrix (M) (ORF4) (287 aa), glycoprotein (G) (ORF5) (520 aa), and an RNA-dependent RNA polymerase (L) (ORF6) (114 aa), as well as a putative movement protein (P3) (ORF3) (189 aa) and the hypothetical small protein P4. The predicted BaCV proteins were compared to homologous proteins from the closest cytorhabdoviruses, and a low level of sequence identity (15?39%) was observed. The phylogenetic analysis shows that BaCV clustered with yerba mate chlorosis-associated virus (YmCaV) and rice stripe mosaic virus (RSMV). Overall, our results provide strong evidence that BaCV is indeed a new virus species in the genus Cytorhabdovirus (family Rhabdoviridae), the first rhabdovirus to be identified infecting common bean.
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectRhabdovirus
dc.titleDouble-stranded RNA high-throughput sequencing reveals a new Cytorhabdovirus in a bean golden mosaic virus-resistant common bean transgenic line.
dc.typeArtigo de periódico
dc.date.updated2020-02-12T11:11:11Zpt_BR
dc.subject.thesagroFeijão
dc.subject.thesagroPhaseolus Vulgaris
dc.subject.thesagroOrganismo Transgênico
dc.subject.thesagroVírus
dc.subject.thesagroMosaico Dourado
dc.subject.nalthesaurusBeans
dc.subject.nalthesaurusCytorhabdovirus
dc.subject.nalthesaurusDsRNA viruses
dc.subject.nalthesaurusTransgenic plants
dc.subject.nalthesaurusBean golden mosaic virus
riaa.ainfo.id1104582
riaa.ainfo.lastupdate2020-02-12 -02:00:00
dc.identifier.doi10.3390/v11010090
dc.contributor.institutionDIONE M. T. ALVES-FREITAS; BRUNA PINHEIRO-LIMA; JOSIAS CORREA DE FARIA, CNPAF; CRISTIANO CASTRO LACORTE, Cenargen; SIMONE DA GRACA RIBEIRO, Cenargen; FERNANDO L. MELO, UNB.
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