Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1106085
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dc.contributor.authorBANDEIRA, A. M. P.
dc.contributor.authorMATOS, J. E.
dc.contributor.authorMARIA, A. N.
dc.contributor.authorCARNEIRO, P. C. F.
dc.contributor.authorPURDY, P. H.
dc.contributor.authorAZEVEDO, H. C.
dc.date.accessioned2019-02-15T23:48:16Z-
dc.date.available2019-02-15T23:48:16Z-
dc.date.created2019-02-15
dc.date.issued2018
dc.identifier.citationAnimal Reproduction, v. 15, n. 1, p. 23-28, Jan./Mar. 2018.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1106085-
dc.descriptionThe physical and chemical characteristics of gelatin have been used to justify its inclusion in extenders to preserve the sperm quality and improve results of cervical artificial insemination with cooled semen. The objective of this study was to evaluate the effects of gelatin supplementation in cooling extender on the quality and fertility of ram semen stored at 5°C. Semen samples (n = 24) of Santa Inês rams (n = 6) were diluted in Glycine-Yolk-Milk extender without (control) or with 1.5% of gelatin. The samples were loaded into 0.25 mL straws, cooled to 5°C and stored vertically for 48 and 72 hours. Sample quality was evaluated using straw homogeneity tests based on pH, osmolality and the proportion of spermatozoa (PS) in both upper and lower segments of straws (US and LS), analyses of sperm motility, plasma and acrosomal membrane integrity, and by fertility after artificial insemination. Differences between the US and LS of straws were found for pH and PS (%). They were significant only in the control group at both times: pH ? 5.96 vs. 5.71 at 48 h and 6.13 vs. 5.89 at 72 h; PS ? 21.66 vs. 78.34 at 48 h and 20.87 vs. 79.13 at 72 h. Storage in gelatin had very little, to no effect on the sperm kinetics or on the sperm membrane integrity evaluations. The addition of gelatin to the extender did not affect the pregnancy rate which ranged from 4.4 to 26.1%. We conclude that gelatin is effective in maintaining the physical and chemical homogeneity of the semen samples. Further research is needed in order to optimize the use of gelatin supplementation and elucidate any potential benefits.
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleThe effects of gelatin supplementation prior to cooling on ram semen quality and fertility.
dc.typeArtigo de periódico
dc.date.updated2019-02-15T23:48:16Zpt_BR
dc.subject.thesagroSêmen
dc.subject.thesagroFertilidade Animal
dc.subject.nalthesaurusAnimal fertility
riaa.ainfo.id1106085
riaa.ainfo.lastupdate2019-02-15
dc.identifier.doi10.21451/1984-3143-AR2017-0021
dc.contributor.institutionANDERSON MARQUES PINTO BANDEIRA, UFS; JOSE EDUARDO MATOS, UFS; ALEXANDRE NIZIO MARIA, CPATC; PAULO CESAR FALANGHE CARNEIRO, CPATC; PHILLIP H. PURDY, ARS; HYMERSON COSTA AZEVEDO, CPATC.
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