Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1107167
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dc.contributor.authorSILVA, P. L. R.eng
dc.contributor.authorMACEDO, L. L. P. deeng
dc.contributor.authorSÁ, M. E. L. deeng
dc.contributor.authorAMORIM. R. M. S. deeng
dc.contributor.authorMORGANTE, C. V.eng
dc.contributor.authorTESSUTTI, I. T. L.eng
dc.contributor.authorBASSO, M. F.eng
dc.contributor.authorGALBIERI, R.eng
dc.contributor.authorSÁ, M. F. G. deeng
dc.date.accessioned2019-03-19T00:47:28Z-
dc.date.available2019-03-19T00:47:28Z-
dc.date.created2019-03-18
dc.date.issued2018
dc.identifier.citationIn: BRAZILIAN BIOTECHNOLOGY CONGRESS, 7.; BIOTECHNOLOGY IBERO-AMERICAN CONGRESS, 2., 2018, Brasília, DF. Proceedings... Brasília, DF: SBBiotec, 2018.eng
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1107167-
dc.descriptionCotton (Gossypium hirsutum) is one of the most important commodities worldwide, and it is among the ten largest sources of wealth in the Brazilian agribusiness. However, its productivity is constantly threatened by biotic and abiotic factors. Plant-parasitic nematodes (PPN) are one of the most damaging pathogens, representing global losses of approximately US$ 100 bi/annual. More and more, biotechnology tools have contributed to overcoming these constraints through the development of tolerant or resistant plants. Interfering RNA (RNAi) technology has been successfully exploited in different plant species for achieving resistance against different pathogens. The cross-talk of these engineered RNAi produced in planthas been shown to be an efficient strategy to knockdown essential genes in PPN. Previous studies of our research team have identified several potential molecules from nematodes involved in nematode-plant interaction. The Mi-1, Mi-2 and Mi-3 genes are, respectively, an avirulence protein, a cysteine protease involved in plant parasitism, and a protein involved in the formation of egg mass matrix. Here, cotton GM plants constitutively overexpressing a hairpin-derived dsRNA targeting simultaneously these three genes of Meloidogyne incognita were obtained from biolistic-transformation. Transgenic plants were in vitro and ex vitro (leaf-painting) selected using herbicide Imazapyr and QuickStix kit for LibertyLink® (bar gene).Twelve PCR-positive events were characterized and plants from T1 and T2 generation were challenged with M. incognita, using root gall index and number of eggs to evaluate plant resistance/susceptibility. Data will be significant to the nematode management in cotton crops.eng
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectAlgodão transgenicoeng
dc.subjectFitonematodeeng
dc.subjectNemátodos parasitas de plantaseng
dc.subjectInterferência de RNAeng
dc.subjectKnockdown de geneseng
dc.titleTransgenic cotton applied to phytonematode control using in plant RNA interfering strategy.eng
dc.typeResumo em anais e proceedingseng
dc.date.updated2019-03-19T00:47:28Z
dc.subject.thesagroAlgodãoeng
dc.subject.thesagroNematóideeng
dc.subject.thesagroGossypium Hirsutumeng
dc.subject.nalthesaurusCottoneng
riaa.ainfo.id1107167eng
riaa.ainfo.lastupdate2019-03-18
dc.contributor.institutionPAOLO L. RODRIGUES SILVA; LEONARDO L. P. DE MACEDO; M. EUGENIA LISEI DE SÁ; REGINA, M. S. DE AMORIM; CAROLINA VIANNA MORGANTE, CPATSA; ISABELA T. LOURENÇO TESSUTTI; MARCOS F. BASSO; RAFAEL GALBIERI; MARIA F. GROSSI DE SÁ.eng
Aparece nas coleções:Resumo em anais de congresso (CPATSA)

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