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dc.contributor.authorFALAVIGNA, V. da S.eng
dc.contributor.authorMALABARBA, J.eng
dc.contributor.authorSILVEIRA, C. P.eng
dc.contributor.authorBUFFON, V.eng
dc.contributor.authorMARIATH, J. E. de A.eng
dc.contributor.authorPASQUALI, G.eng
dc.contributor.authorMARGIS-PINHEIRO, M.eng
dc.contributor.authorREVERS, L. F.eng
dc.date.accessioned2019-06-12T00:39:01Z-
dc.date.available2019-06-12T00:39:01Z-
dc.date.created2019-06-11
dc.date.issued2019
dc.identifier.citationPlant Cell Reports [online], 2019.eng
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1109788-
dc.descriptionAbstract Key message MdoDHN11 acts in the nucellus layer to protect the embryo and the endosperm from limited water availability during apple seed development.AbstractDehydrins (DHNs) are protective proteins related to several plant developmental responses that involve dehy-dration such as seed desiccation and abiotic stresses. In apple (Malus × domestica Borkh.), the seed-specific MdoDHN11was suggested to play important roles against dehydration during seed development. However, this hypothesis has not yet been evaluated. Within this context, several experiments were performed to functionally characterize MdoDHN11. In situ hybridization analysis during apple seed development showed that MdoDHN11 expression is confined to a maternal tissue called nucellus, a central mass of parenchyma between the endosperm and the testa. The MdoDHN11 protein was local-ized in the cytosol and nucleus. Finally, transgenic Arabidopsis plants expressing MdoDHN11 were generated and exposed to a severe water-deficit stress, aiming to mimic a situation that can occurs during seed development. All transgenic lines showed increased tolerance to water deficit in relation to wild-type plants. Taken together, our results provide evidences that MdoDHN11 plays important roles during apple seed development by protecting the embryo and the endosperm from limited water availability, and the mechanism of action probably involves the interaction of MdoDHN11 with proteins and other components in the cell.Keywords Apple · Dehydrin · Seed development · Transgenic expression · Water-deficit stresseng
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectTransgenic expressioneng
dc.subjectWater-deficit stresseng
dc.titleCharacterization of the nucellus-specific dehydrin MdoDHN11 demonstrates its involvement in the tolerance to water deficit.eng
dc.typeArtigo de periódicoeng
dc.date.updated2020-01-06T11:11:11Z
dc.subject.nalthesaurusAppleseng
dc.subject.nalthesaurusDehydrinseng
dc.subject.nalthesaurusSeed developmenteng
riaa.ainfo.id1109788eng
riaa.ainfo.lastupdate2020-01-06 -02:00:00
dc.identifier.doi10.1007/s00299-019-02428-8eng
dc.contributor.institutionVítor da Silveira Falavigna, Graduate Program in Cell and Molecular Biology, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91501-970, Brazil; Jaiana Malabarba, Graduate Program in Cell and Molecular Biology, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91501-970, BraziL; Carolina Pereira Silveira, Embrapa Uva e Vinho, Rua Livramento, 515, P.O. Box 130, Bento Gonçalves, RS 95701-008, Brazil; VANESSA BUFFON, CNPUV; Jorge Ernesto de Araújo Mariath, Laboratory of Plant Anatomy, Botany Department, UFRGS, Porto Alegre 91501-970, Brazil; Giancarlo Pasquali, Graduate Program in Cell and Molecular Biology, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91501-970, Brazil; Márcia Margis?Pinheiro, Graduate Program in Cell and Molecular Biology, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91501-970, Brazil; LUIS FERNANDO REVERS, CNPUV.eng
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