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dc.contributor.authorBOTTA, D.
dc.contributor.authorARRUDA, R. P. de
dc.contributor.authorWATANABE, Y. F.
dc.contributor.authorBALIEIRO, J. C. de C.
dc.contributor.authorROMANELLO, N.
dc.contributor.authorBARRETO, A. do N.
dc.contributor.authorPANTOJA, M. H. de A.
dc.contributor.authorGIRO, A.
dc.contributor.authorCARVALHO, C. P. T. de
dc.contributor.authorOLIVEIRA, A. de S.
dc.contributor.authorGARCIA, A. R.
dc.date.accessioned2023-04-12T13:43:28Z-
dc.date.available2023-04-12T13:43:28Z-
dc.date.created2019-06-19
dc.date.issued2019
dc.identifier.citationAndrologia, v. 51, n. 6, e13266, jul. 2019.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1109972-
dc.descriptionOur aim was to evaluate the effects of three thermal environments over time on kinetics, functionality and in vitro fertility of cryopreserved bovine spermatozoa. Four ejaculates from five bulls (n = 20) were cryopreserved. After thawing, semen was evaluated (0 hr), incubated for 4 hr in T36.0 (36.0°C), T38.0 (38.0°C) and T39.5 (39.5°C), and analysed every hour (1 hr, 2 hr, 3 hr, 4 hr). In vitro production of embryos was performed at 0 hr and 4 hr. Sperm motility and cell kinetics (Computer Assisted Sperm Analysis) were impaired after 2 hr at T38.0 and T39.5 (p < 0.05). Flow cytometry revealed an increase in the cells with injured plasma membrane to 39.5°C and a general reduction in the mitochondrial potential over time (p < 0.05). In vitro fertility was impaired in all temperatures after 4 hr, but there was no difference between 36.0°C and 38.0°C. Our results suggest that the ex situ resilience of semen at 36.0°C after thawing with no major damage to the quality is limited to 3 hr. In normothermia or in thermal stress, sperm cells present a gradual reduction of movement and functionality, which were more significant after 1 hr of incubation. The in vitro production of embryos is impaired when the semen is kept in a thermal environment &#8805;36.0°C for 4 hr.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectSemen quality
dc.subjectThermotolerance
dc.titleInfluence of post-thawing thermal environment on bovine sperm characteristics and in vitro fertility.
dc.typeArtigo de periódico
dc.subject.thesagroTouro
dc.subject.thesagroInseminação Artificial
dc.subject.nalthesaurusBulls
dc.subject.nalthesaurusIn vitro fertilization
dc.subject.nalthesaurusHeat stress
riaa.ainfo.id1109972
riaa.ainfo.lastupdate2023-03-13
dc.identifier.doi10.1111/and.13266
dc.contributor.institutionDaniela Botta, UFPA; Rubens Paes de Arruda, USP; Yeda Fumie Watanabe, Vitrogen - YVF Biotech; Júlio Cesar de Carvalho Balieiro, USP; Narian Romanello, UFPA; Andréa do Nascimento Barreto, UFPA; Messy Hannear de Andrade Pantoja, UFPA; Alessandro Giro, UFPA; Carla Patrícia Teodoro de Carvalho, USP; Aline de Sousa Oliveira, Vitrogen - YVF Biotech; ALEXANDRE ROSSETTO GARCIA, CPPSE.
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