Por favor, use este identificador para citar o enlazar este ítem: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1109972
Registro completo de metadatos
Campo DCValorLengua/Idioma
dc.contributor.authorBOTTA, D.
dc.contributor.authorARRUDA, R. P. de
dc.contributor.authorWATANABE, Y. F.
dc.contributor.authorBALIEIRO, J. C. de C.
dc.contributor.authorROMANELLO, N.
dc.contributor.authorBARRETO, A. do N.
dc.contributor.authorPANTOJA, M. H. de A.
dc.contributor.authorGIRO, A.
dc.contributor.authorCARVALHO, C. P. T. de
dc.contributor.authorOLIVEIRA, A. de S.
dc.contributor.authorGARCIA, A. R.
dc.date.accessioned2023-04-12T13:43:28Z-
dc.date.available2023-04-12T13:43:28Z-
dc.date.created2019-06-19
dc.date.issued2019
dc.identifier.citationAndrologia, v. 51, n. 6, e13266, jul. 2019.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1109972-
dc.descriptionOur aim was to evaluate the effects of three thermal environments over time on kinetics, functionality and in vitro fertility of cryopreserved bovine spermatozoa. Four ejaculates from five bulls (n = 20) were cryopreserved. After thawing, semen was evaluated (0 hr), incubated for 4 hr in T36.0 (36.0°C), T38.0 (38.0°C) and T39.5 (39.5°C), and analysed every hour (1 hr, 2 hr, 3 hr, 4 hr). In vitro production of embryos was performed at 0 hr and 4 hr. Sperm motility and cell kinetics (Computer Assisted Sperm Analysis) were impaired after 2 hr at T38.0 and T39.5 (p < 0.05). Flow cytometry revealed an increase in the cells with injured plasma membrane to 39.5°C and a general reduction in the mitochondrial potential over time (p < 0.05). In vitro fertility was impaired in all temperatures after 4 hr, but there was no difference between 36.0°C and 38.0°C. Our results suggest that the ex situ resilience of semen at 36.0°C after thawing with no major damage to the quality is limited to 3 hr. In normothermia or in thermal stress, sperm cells present a gradual reduction of movement and functionality, which were more significant after 1 hr of incubation. The in vitro production of embryos is impaired when the semen is kept in a thermal environment &#8805;36.0°C for 4 hr.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectSemen quality
dc.subjectThermotolerance
dc.titleInfluence of post-thawing thermal environment on bovine sperm characteristics and in vitro fertility.
dc.typeArtigo de periódico
dc.subject.thesagroTouro
dc.subject.thesagroInseminação Artificial
dc.subject.nalthesaurusBulls
dc.subject.nalthesaurusIn vitro fertilization
dc.subject.nalthesaurusHeat stress
riaa.ainfo.id1109972
riaa.ainfo.lastupdate2023-03-13
dc.identifier.doi10.1111/and.13266
dc.contributor.institutionDaniela Botta, UFPA; Rubens Paes de Arruda, USP; Yeda Fumie Watanabe, Vitrogen - YVF Biotech; Júlio Cesar de Carvalho Balieiro, USP; Narian Romanello, UFPA; Andréa do Nascimento Barreto, UFPA; Messy Hannear de Andrade Pantoja, UFPA; Alessandro Giro, UFPA; Carla Patrícia Teodoro de Carvalho, USP; Aline de Sousa Oliveira, Vitrogen - YVF Biotech; ALEXANDRE ROSSETTO GARCIA, CPPSE.
Aparece en las colecciones:Artigo em periódico indexado (CPPSE)

Ficheros en este ítem:
Fichero Descripción TamañoFormato 
BOTTA-et-al-2019-Influence-of-Post-thawing-Thermal-Environment-on-Bovine-Sperm-Characteristics.pdf827.09 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro sencillo del ítem

FacebookTwitterDeliciousLinkedInGoogle BookmarksMySpace