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| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.contributor.author | GREGORINE, L. F. | eng |
| dc.contributor.author | MONCLARO, A. V. | eng |
| dc.contributor.author | SILVA, C. de O. G. | eng |
| dc.contributor.author | RODRIGUES, K. B. | eng |
| dc.contributor.author | PEIXOTO, J. S. G. | eng |
| dc.contributor.author | ABDELNUR, P. V. | eng |
| dc.contributor.author | FAVARO, L. C. de L. | eng |
| dc.date.accessioned | 2019-09-24T00:40:26Z | - |
| dc.date.available | 2019-09-24T00:40:26Z | - |
| dc.date.created | 2019-09-23 | |
| dc.date.issued | 2019 | |
| dc.identifier.citation | In: SIMPÓSIO NACIONAL DE BIOPROCESSOS, 22.; SIMPÓSIO DE HIDRÓLISE ENZIMÁTICA DE BIOMASSA, 13., 2019, Uberlandia, MG. [Anais ...]. São Paulo: Associação Brasileira de Engenharia Química, 2019. | eng |
| dc.identifier.uri | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1112393 | - |
| dc.description | The powerful class of oxidative enzymes, lytic polysaccharide monooxygenases (LPMOs) - also named Auxiliary Activity (AA) - are able to oxidize recalcitrant polysaccharides on lignocellulosic biomass. In this work, we successfully expressed three catalytic domains from bacterial LPMOs in the yeast Komagataella phaffii: domain MdAA10.1-SD (from Moritella dasanensis), domain VmAA10.2-SD (from Verrucosispora maris), and domain HgAA10.1-SD (from Hahella ganghwensis). Heterologous expression was analyzed by SDS-PAGE, Western-Blot, and Dot-Blot, while functional activity of these proteins was investigated by a combination of mass spectrometric and chromatographic methods. The recombinant LPMO catalytic domain HgAA10.1-SD from H. ganghwensis, a C1-oxidizer, was able to promote an oxidative cleavage of phosphoric-acid swollen cellulose (PASC) substrate in the presence of ascorbic acid as an electron donor, showing its potential for cellulose depolymerization. | eng |
| dc.language.iso | eng | eng |
| dc.rights | openAccess | eng |
| dc.subject | Spectrometric | eng |
| dc.subject | Chromatographic | eng |
| dc.title | Heterologous expression of a new lytic polysaccharide monooxygenase from Hahella ganghwensis and their functional characterization. | eng |
| dc.type | Artigo em anais e proceedings | eng |
| dc.date.updated | 2019-09-24T00:40:26Z | |
| riaa.ainfo.id | 1112393 | eng |
| riaa.ainfo.lastupdate | 2019-09-23 | |
| dc.contributor.institution | Lucas F. Gregorine; Antonielle V. Monclaro; Caio de Oliveira Gorgulho Silva, Consultor da Embrapa Agroenergia; Kelly B. Rodrigues; Jéssica S. G. Peixoto, UnB; PATRICIA VERARDI ABDELNUR, CNPAE; LEIA CECILIA DE LIMA FAVARO, CNPAE. | eng |
| Aparece en las colecciones: | Artigo em anais de congresso (CNPAE)  | |
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| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| 107193786998fieldsubmissionfulltextfile1.pdf | 623.05 kB | Adobe PDF |  Visualizar/Abrir |
