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dc.contributor.authorPORTO, B. N.eng
dc.contributor.authorCAIXETA, E. T.eng
dc.contributor.authorMATHIONI, S. M.eng
dc.contributor.authorVIDIGAL, P. M. P.eng
dc.contributor.authorZAMBOLIM, L.eng
dc.contributor.authorZAMBOLIM, E. M.eng
dc.contributor.authorDONOFRIO, N.eng
dc.contributor.authorPOLSON, S. W.eng
dc.contributor.authorMAIA, T. A.eng
dc.contributor.authorCHEN, C.eng
dc.contributor.authorADETUNJI, M.eng
dc.contributor.authorKINGHAM, B.eng
dc.contributor.authorDALIO, R. J. D.eng
dc.contributor.authorRESENDE, M. L. V. deeng
dc.date.accessioned2019-10-18T18:10:13Z-
dc.date.available2019-10-18T18:10:13Z-
dc.date.created2019-10-18
dc.date.issued2019
dc.identifier.citationPLOSONE, April 18, 2019.eng
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1113216-
dc.descriptionCoffee leaf rust caused by the fungus Hemileia vastatrix is one of the most important leaf diseases of coffee plantations worldwide. Current knowledge of the H. vastatrix genome is limited and only a small fraction of the total fungal secretome has been identified. In order to obtain a more comprehensive understanding of its secretome, we aimed to sequence and assemble the entire H. vastatrix genome using two next-generation sequencing platforms and a hybrid assembly strategy. This resulted in a 547 Mb genome of H. vastatrix race XXXIII (Hv33), with 13,364 predicted genes that encode 13,034 putative proteins with transcriptomic support. Based on this proteome, 615 proteins contain putative secretion peptides, and lack transmembrane domains. From this putative secretome, 111 proteins were identified as candidate effectors (EHv33) unique to H. vastatrix, and a subset consisting of 17 EHv33 genes was selected for a temporal gene expression analysis during infection. Five genes were significantly induced early during an incompatible interaction, indicating their potential role as pre-haustorial effectors possibly recognized by the resistant coffee genotype. Another nine genes were significantly induced after haustorium formation in the compatible interaction. Overall, we suggest that this fungus is able to selectively mount its survival strategy with effectors that depend on the host genotype involved in the infection process.eng
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleGenome sequencing and transcript analysis of Hemileia vastatrix reveal expression dynamics of candidate effectors dependent on host compatibility.eng
dc.typeArtigo de periódicoeng
dc.date.updated2019-10-18T18:10:13Z
dc.subject.thesagroHemileia Vastatrixeng
dc.subject.thesagroFungoeng
dc.subject.thesagroFerrugemeng
dc.subject.nalthesaurusGenomeeng
dc.subject.nalthesaurusLeaf rusteng
dc.subject.nalthesaurusInfectioneng
riaa.ainfo.id1113216eng
riaa.ainfo.lastupdate2019-10-18
dc.contributor.institutionBrenda Neves Porto, Universidade Federal de Lavras - UFLA. Progama de Pós-graduação em Biotecnologia Vegetaleng
dc.contributor.institutionEVELINE TEIXEIRA CAIXETA MOURA, CNPCaeng
dc.contributor.institutionSandra Marisa Mathioni, Universidade Federal de Lavras - UFLA/Departamento de Fitopatologiaeng
dc.contributor.institutionPedro Marcus Pereira Vidigal, Universidade Federal de Viçosa - UFV/Núcleo de Análises de Biomoléculaseng
dc.contributor.institutionLaércio Zambolim, Universidade Federal de Viçosa - UFV/Departamento de Fitopatologiaeng
dc.contributor.institutionEunize Maciel Zambolim, Universidade Federal de Viçosa - UFV/Departamento de Fitopatologiaeng
dc.contributor.institutionNicole Donofrio, University of Delaware/Department of Plant and Soil Scienceseng
dc.contributor.institutionShawn W. Polson, Delaware Biotechnology Institute/Center for Bioinformatics and Computational Biologyeng
dc.contributor.institutionThiago Andrade Maia, Universidade Federal de Viçosa - UFV/Departamento de Fitopatologiaeng
dc.contributor.institutionChuming Chen, Delaware Biotechnology Institute/Center for Bioinformatics and Computational Biologyeng
dc.contributor.institutionModupe Adetunji, Delaware Biotechnology Institute/Center for Bioinformatics and Computational Biologyeng
dc.contributor.institutionBrewster Kingham, University of Delaware, Newark/Delaware Biotechnology Institute/Sequencing and Genotyping Centereng
dc.contributor.institutionRonaldo José Durigan Dalio, Instituto Agronoˆmico de Campinas - IAC/Centro de Citricultura “Sylvio Moreira”eng
dc.contributor.institutionMário Lúcio Vilela de Resende, Departamento de Fitopatologia, Universidade Federal de Lavras.eng
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