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dc.contributor.authorAMARANTE, J. F.eng
dc.contributor.authorRIBEIRO, M. de F.eng
dc.contributor.authorCOSTA, M. M.eng
dc.contributor.authorMENEZES, F. G.eng
dc.contributor.authorSILVA, T. M. Seng
dc.contributor.authorAMARANTE, T. A. B.eng
dc.contributor.authorGRADELA, A.eng
dc.contributor.authorMOURA, L. M. D.eng
dc.date.accessioned2019-11-08T18:15:44Z-
dc.date.available2019-11-08T18:15:44Z-
dc.date.created2019-11-08
dc.date.issued2019
dc.identifier.citationPesquisa Veterinária Brasileira, v. 39, n. 9, p. 734-743, 2019.eng
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1114052-
dc.descriptionThere is a growing need to discover and develop alternative therapies for the treatment of mastitis caused by Staphylococcus spp. and multidrug-resistant bacterial infections. This study examined the chemical composition and antimicrobial potential of two propolis extracts (EPA and EPB) against seventy-seven isolates of Staphylococcus spp. obtained from subclinical bovine mastitis; three clinical strains of MRSA and two from clinical strains of S. aureus ATCC, identified as S. aureus ATCC 6538 and S. aureus ATCC 25923. The total phenolic content was determined by the Folin-Ciocalteau method, the total flavonoid content by the Dowd method and the phenolic profile was quantified by HPLC-DAD. The MBC values of the extracts were evaluated by broth microdilution method. The amount of total phenolic and flavonoid compounds was higher in EPA than EPB. Both extracts revealed the presence of caffeic, coumaric, cinnamic, ferulic and 3,4-dihydroxybenzoic acids, with higher concentrations of coumaric and cinnamic acids. Staphylococcus spp. isolates were susceptible to EPA (90.9%), EPB (83.1%) and oxacillin (80.5%). The oxacillin susceptible isolates were also susceptible to EPA (70.1%) and EPB (80.6%), whereas those oxacillin-resistant strains were also susceptible to EPA (40.0%) and to EPB (26.7%). MBC ranged from 34.3 to 68.7?m/mL for EPA and from 68.7 to 137.5?g/mL for EPB. Both extracts inhibited significantly (100%) the clinical strains of MRSA, S. aureus ATCC 6538 and S. aureus ATCC 25923 at the concentration of 68.7?g/mL. It is concluded that both extracts of propolis, whose main constituents are coumaric and cinnamic acids, have high antimicrobial activity against the microorganisms studied, and EPA also against oxacillin-resistant strains. These findings reinforce its potential use for the treatment of bovine mastitis.eng
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectMastite bovinaeng
dc.subjectAtividade antimicrobianaeng
dc.subjectExtrato de própoliseng
dc.subjectTerapia alternativaeng
dc.titleChemical composition and antimicrobial activity of two extract of propolis against isolates of Staphylococcus spp. and multiresistant bacterials.eng
dc.typeArtigo de periódicoeng
dc.date.updated2019-11-08T18:15:44Z
dc.subject.thesagroComposição Químicaeng
dc.subject.thesagroPrópoliseng
dc.subject.thesagroDoença Animaleng
dc.subject.thesagroBactériaeng
dc.subject.nalthesaurusStaphylococcus aureus subsp. aureuseng
dc.subject.nalthesaurusAnimal diseaseseng
riaa.ainfo.id1114052eng
riaa.ainfo.lastupdate2019-11-08
dc.identifier.doi10.1590/1678-5150-PVB-6128eng
dc.contributor.institutionJarbas F. Amarante, UNIVASF; MARCIA DE FATIMA RIBEIRO, CPATSA; Mateus M. Costa, UNIVASF; Fredson G. Menezes, UNIVASF; Tania M. S. Silva, UFRPE; Talita A. B. Amarante, UNEB; Adriana Gradela, UNIVASF; Liliane M. D. Moura, UNIVASF.eng
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