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dc.contributor.authorROJAS-LORZ, L.eng
dc.contributor.authorARRIETA-ESPINOZA, G.eng
dc.contributor.authorVALDEZ-MELARA, M.eng
dc.contributor.authorPEREIRA, L. F. P.eng
dc.contributor.authorGATICA-ARIAS, A.eng
dc.date.accessioned2019-12-09T18:11:23Z-
dc.date.available2019-12-09T18:11:23Z-
dc.date.created2019-12-09
dc.date.issued2019
dc.identifier.citationBrazilian Archives of Biology and Technology, v. 62, e19180228, 2019.eng
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1116284-
dc.descriptionPlant somatic embryogenesis (SE) has been defined as the formation of embryos from a single or group of haploid or somatic cells [1, 2]. Low frequency (LFSE) and high frequency somatic embryogenesis (HFSE) have been described. In the first type, somatic embryos are induced directly from pro-embryogenic cells of explants, while in the second, they originate from embryogenic callus [1]. It has been suggested that in LFSE the origin of somatic embryos is unicellular, whereas in HFSE has been described as unicellular or multicellular [3]. SE is a powerful biotechnological tool used to propagate elite plants or to conserve important genotypes [4]. Moreover, SE offers an efficient in vitro regeneration approach as a fundamental step in plant genetic improvement for studying basic aspects of ontogenesis of somatic embryos [5]. In Coffea spp., the first studies of SE have been reported at the beginning of 1970 [6]. Since then, a large quantity of LFSE and HFSE protocols have been optimized demonstrating that coffee is not a recalcitrant species for SE [4]. In the LFSE the somatic embryos are obtained faster (approximately 70 days) using only one medium meanwhile in HFSE several media are used and somatic embryo formation takes 9-10 months [4]. Although, in LFSE small number of somatic embryos are obtained (around 10 per explant) compared to hundreds of somatic embryos obtained per gram of embryogenic calli [4], the unicellular origin of somatic embryos in LFSE represents an advantage for the chemical and physical mutagenesis, genetic transformation and genetic editing, since prevents or reduces the appearance of chimeras [7]. In C. arabica and C. canephora many factors (such as genotype, explant type, the physiological state, age and growth conditions of the donor plants, the season of collection, nutrient composition of the medium, the volume of dissolved CO2 or O2 in the culture flask, and plant growth regulators) that affect LFSE induction have been studied [3, 8, 9, 10, 11, 12, 13]. However, few studies reported the effect of silver nitrate on LFSE using leaf explants of C. arabica L. and to the best of our knowledge it has not been analyzed using Caturra and Catuaí, which are two economic important producer cultivars in Costa Rica. Since SE is genotype dependent, the culture medium need to be modified for the different genotypes [7].Therefore, the objective of this study was to determine the influence of the benzyladenine (BAP), indole-3-acetic acid (IAA), and silver nitrate (AgNO3) on low frequency somatic embryogenesis using leaf explants of Coffea arabica L. cultivars Caturra and Catuaí.eng
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectPlant growth regulatorseng
dc.titleInfluence of Silver Nitrate on Somatic Embryogenesis Induction in Arabica Coffee (Coffea arabica L.).eng
dc.typeArtigo de periódicoeng
dc.date.updated2019-12-13T11:11:11Z
dc.subject.nalthesaurusSilver nitrateeng
dc.subject.nalthesaurusSomatic embryoseng
dc.subject.nalthesaurusTissue cultureeng
riaa.ainfo.id1116284eng
riaa.ainfo.lastupdate2019-12-13 -02:00:00
dc.contributor.institutionLaura Rojas-Lorz, University of Costa Rica/School of Biology/Laboratory of Plant Biotechnologyeng
dc.contributor.institutionGriselda Arrieta-Espinoza, University of Costa Rica/Research Center in Cellular and Molecular Biology - CIBCMeng
dc.contributor.institutionMarta Valdez-Melara, University of Costa Rica/School of Biology/Laboratory of Plant Biotechnologyeng
dc.contributor.institutionLUIZ FILIPE PROTASIO PEREIRA, CNPCaeng
dc.contributor.institutionAndrés Gatica-Arias, University of Costa Rica/School of Biology/Laboratory of Plant Biotechnology.eng
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