Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1116519
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dc.contributor.authorCARVALHO, B. P.eng
dc.contributor.authorCOSTA, F. de Q.eng
dc.contributor.authorDETONI, D.eng
dc.contributor.authorROSA, F. B.eng
dc.contributor.authorDIAS, A. J. B.eng
dc.date.accessioned2019-12-10T18:30:00Z-
dc.date.available2019-12-10T18:30:00Z-
dc.date.created2019-12-10
dc.date.issued2019
dc.identifier.citationRevista Brasileira de Zootecnia, v. 48, e20180322, set. 2019.eng
dc.identifier.issn1806-9290eng
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1116519-
dc.descriptionThis study evaluated the effect of the addition of conjugated linoleic acid (CLA) to in vitro culture on viability, lipid content, and cryoresistance of bovine embryos at different in vitro culture times. Cumulus oocyte complexes (N = 974) were maturated in vitro for 22 h. In vitro fecundation ensued for 18 h. Viable zygotes were cultivated in vitro in medium supplemented with CLA (100 mM) in the first 72 h (CLA-F), last 72 h (CLA-L), or throughout the culture period (CLA-T). Control embryos (control) were cultivated with no CLA. Embryos were cryopreserved by vitrification for subsequent analysis after devitrification. Effect of CLA on cryoresistance was assessed by cultivating embryos in synthetic oviductal fluid containing 5% fetal bovine serum. Lipid content was quantified using Nile Red staining. No significant difference was observed in cleavage rate, blastocyst:total oocyte ratio, and blastocyst:cleaved oocyte ratio. Culture in CLA-L reduced survival rate 24 h after devitrification compared with CLA-F and CLA-T, although with no statistically significant difference compared with control group. However, CLA-T improved embryo hatching rate and affected lipid content of embryos. Cultures CLA-F and CLA-L increased lipid content compared with control. Yet, lipid content values decreased in embryos treated with CLA-T, but they did not differ significantly from the values observed for oocytes at the germinal vesicle stage. Treatment of bovine embryos with CLA during in vitro cultivation did not affect the production of blastocysts, reducing lipid content and improving cryoresistance. However, the effects of CLA on cryoresistance and lipid content is significant only when embryos are exposed to the compound throughout the cultivation period.eng
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectGanado bovinoeng
dc.subjectReproducción de animaleseng
dc.subjectEmbrión (animal)eng
dc.subjectCultivo de embrioneseng
dc.subjectContenido de lípidoseng
dc.subjectÁcido linoleíco conjugadoeng
dc.subjectÁcido graxo poliinsaturadoeng
dc.subjectÁcidos grasos polisaturadoseng
dc.subjectOocyte vitrificationeng
dc.subjectVitrificación de ovocitoseng
dc.subjectCriopreservacióneng
dc.subjectTolerancia al fríoeng
dc.titleUse of conjugated linoleic acid (trans 10, cis 12) to cultivate bovine embryos: effect on cryoresistance and lipid content.eng
dc.typeArtigo de periódicoeng
dc.date.updated2019-12-10T18:30:00Z
dc.subject.thesagroBovinoeng
dc.subject.thesagroReprodução Animaleng
dc.subject.thesagroEmbrião Animaleng
dc.subject.thesagroCultura In Vitroeng
dc.subject.thesagroLipídioeng
dc.subject.thesagroCriopreservaçãoeng
dc.subject.thesagroResistência a Temperaturaeng
dc.subject.thesagroCongelamentoeng
dc.subject.nalthesaurusCattleeng
dc.subject.nalthesaurusAnimal reproductioneng
dc.subject.nalthesaurusEmbryo (animal)eng
dc.subject.nalthesaurusEmbryo cultureeng
dc.subject.nalthesaurusLipid contenteng
dc.subject.nalthesaurusConjugated linoleic acideng
dc.subject.nalthesaurusPolyunsaturated fatty acidseng
dc.subject.nalthesaurusCryopreservationeng
dc.subject.nalthesaurusCold toleranceeng
riaa.ainfo.id1116519eng
riaa.ainfo.lastupdate2019-12-10
dc.identifier.doi10.1590/rbz4820180322eng
dc.contributor.institutionBRUNO PENA CARVALHO, CPAF-AC; Fernanda de Queirós Costa, Universidade Estadual do Norte Fluminense Darcy Ribeiro; Danielly Detoni, Universidade Estadual do Norte Fluminense Darcy Ribeiro; Felipe Borges Rosa, Universidade Estadual do Norte Fluminense Darcy Ribeiro; Angelo José Burla Dias, Universidade Estadual do Norte Fluminense Darcy Ribeiro.eng
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