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dc.contributor.authorSOUZA, I. I. F. deeng
dc.contributor.authorRODRIGUES, R. de A.eng
dc.contributor.authorJORGE, K. S. G.eng
dc.contributor.authorSILVA, M. R.eng
dc.contributor.authorLILENBAUM, W.eng
dc.contributor.authorVIDAL, C. E. S.eng
dc.contributor.authorETGES, R. N.eng
dc.contributor.authorKOSTOVIC, M.eng
dc.contributor.authorARAUJO, F. R.eng
dc.date.accessioned2019-12-28T00:38:39Z-
dc.date.available2019-12-28T00:38:39Z-
dc.date.created2019-12-27
dc.date.issued2019
dc.identifier.citationThe Journal Veterinary Medical Science, v. 81, n. 1, p. 9-14, 2019.eng
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1117817-
dc.descriptionBovine tuberculosis (bTB) control programs generally rely on intradermal tuberculin tests for the antemortem diagnosis of Mycobacterium bovis infection in cattle, but these tests detect only a portion of the infected animals. The aim of the present study was to evaluate the diagnostic coverage of a combination of the bTB antemortem techniques known as the comparative intradermal tuberculin test (CITT) and an ELISA based on a recombinant chimera of ESAT-6/MPB70/MPB83 as the antigen in cattle. The results were compared to postmortem findings based on M. bovis culturing and PCR. Paired comparisons of all data (n=92) demonstrated that ELISA and LST results compared to the culturing results did not present significant differences (P=0.27 on McNemar?s test and P=0.12 on Fisher?s exact test, respectively). Using culturing as the gold standard, the sensitivity and specificity of ELISA were 79.5% (95% CI: 64.5?89.2%) and 75.5% (95% CI: 62.4?85.1%), respectively, whereas LST demonstrated 100% sensitivity (95% CI: 91.03? 100%) and 92.5% specificity (95% CI: 82.1?97.0%). The ELISA results did not reveal significant differences in relation to the LST results (P>0.99 on Fisher?s exact test). Using the latter as the gold standard, the sensitivity and specificity of ELISA were 79.1% (95% CI: 64.8?88.6%) and 79.6% (95% CI: 66.4?88.5%), respectively. The use of ELISA with the recombinant chimera of ESAT-6/MPB70/ MPB83 as the antigen complements the diagnostic coverage provided by CITT and increases the removal of infected animals from herds.eng
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectComparative intradermal tuberculin testeng
dc.titleELISA using a recombinant chimera of ESAT-6/MPB70/MPB83 for Mycobacterium bovis diagnosis in naturally infected cattle.eng
dc.typeArtigo de periódicoeng
dc.date.updated2019-12-28T00:38:39Z
dc.subject.nalthesaurusBovine tuberculosiseng
dc.subject.nalthesaurusMycobacterium bovis BCGeng
riaa.ainfo.id1117817eng
riaa.ainfo.lastupdate2019-12-27
dc.contributor.institutionIngrid Ieda Fernando de Souza, Universidade Federal de Mato Grosso do Sul - UFMS; Rudielle de Arruda Rodrigues, Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Medicina, Veterinaria e Zootecnia- FMVZ; Kláudia Santos Gonçalves Jorge, Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Medicina, Veterinaria e Zootecnia- FMVZ; MARCIO ROBERTO SILVA, CNPGL; Walter Lilenbaum, Universidade Federal Fluminense - UFLU/Laboratório de Bacteriologia e Veterinária; Carlos Eugênio Soto Vidal; Rodrigo Nestor Etges; Miladin Kostovic; FLABIO RIBEIRO DE ARAUJO, CNPGC.eng
Aparece en las colecciones:Artigo em periódico indexado (CNPGC)

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