Title:	Matrix Assisted Laser Desorption Ionization-Time-of-Flight mass spectrometry identification of Mycobacterium bovis in Bovinae.
Authors:	BACANELLI, G. OLARTE, L. C. SILVA, M. R. RODRIGUES, R. A. CARNEIRO, P. A. M. KANEENE, J. B. PASQUATTI, T. N. TAKATANI, H. ZUMÁRRAGA, M. J. ETGES, R. N. ARAUJO, F. R. VERBISCK, N. V.
Affiliation:	GISELE BACANELLI, Universidade Federal de Mato Grosso do Sul - UFMS/Programa de Pós-Graduação em Biotecnologia e Biodiversidade da Região Centro-Oeste LARISSA C. OLARTE, Universidade Federal de Mato Grosso do Sul - UFMS/Programa de Pós-Graduação Multicêntrica em Bioquímica e Biologia Molecular MARCIO ROBERTO SILVA, CNPGL RUDIELLE A. RODRIGUES, Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Medicina Veterinária/Programa de Pós-Graduação em Ciências Veterinárias PAULO A. M. CARNEIRO, Michigan State University/Center for Comparative Epidemiology JOHN B. KANEENE, Michigan State University/Center for Comparative Epidemiology TAYNARA N. PASQUATTI, Universidade Católica Dom Bosco - UCDB HARUO TAKATANI, Agência de Defesa Agrícola do Amazonas MARTIN J. ZUMÁRRAGA, Institute of Biotechnology, CICVyA/INTA RODRIGO N. ETGES, Secretário de Agricultura, Pecuária e Irrigação de Porto Alegre FLABIO RIBEIRO DE ARAUJO, CNPGC NEWTON VALERIO VERBISCK, CNPGC.
Date Issued:	2019
Citation:	Journal the Veterinary Medical Science, v. 81, n. 10, p.1400?1408, 2019.
Description:	In this study, Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDITOF) mass spectrometry was used to identify Mycobacterium bovis from cattle and buffalo tissue isolates from the North and South regions of Brazil, grown in solid medium and previously identified by Polymerase Chain Reaction (PCR) based on Region of Difference 4 (RD4), sequencing and spoligotyping. For this purpose, the protein extraction protocol and the mass spectra reference database were optimized for the identification of 80 clinical isolates of mycobacteria. As a result of this optimization, it was possible to identify and differentiate M. bovis from other members of the Mycobacterium tuberculosis complex with 100% specificity, 90.91% sensitivity and 91.25% reliability. MALDI-TOF MS methodology described herein provides successful identification of M. bovis within bovine/bubaline clinical samples, demonstrating its usefulness for bovine tuberculosis diagnosis in the future.
NAL Thesaurus:	Bovine tuberculosis Mycobacterium bovis BCG Mycobacterium tuberculosis complex
Keywords:	Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPGC)