Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1118013
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dc.contributor.authorANDRADE, A. P. C. DE
dc.contributor.authorBORGES, M. DE F.
dc.contributor.authorEVÂNIA ALTINA TEIXEIRA DE FIGUEIREDO
dc.contributor.authorARCURI, E. F.
dc.date.accessioned2025-05-22T12:47:49Z-
dc.date.available2025-05-22T12:47:49Z-
dc.date.created2020-01-02
dc.date.issued2019
dc.identifier.citationBoletim Centro de Pesquisa de Processamento de Alimentos, Curitiba, v. 36, n. 1, p. 1-9, jan./jun. 2019.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1118013-
dc.descriptionThree hundred samples of coalho cheese, from 15 diff erent brands, seven handmade and eight industrialized, were evaluated in relation to the contamination profi le by Staphylococcus coagulase-positive and negative and the occurrence of staphylococcal enterotoxin encoding genes. Two hundred and eight isolates of Staphylococcus sp. were subjected to phenotypic identifi cation and 95 were subjected to genotypic identifi cation through femA gene research and detection of genes (sea, seb, sec, sed, see, seg, seh, sei and sej) encoding enterotoxins, using the polymerase chain reaction technique (PCR). A total of 14 species of Staphylococcus were identifi ed, of which three were coagulase-positive and eleven negative, especially: S. aureus, S. xylosus, S. cohnni spp. cohnii, S. saprophyticus, S. epidermidis, S. hyicus, S. lentus, S. sciuri, S. cohnii spp. urealyticus, S. haemolyticus, S. chromogenes, S. lugdunensis, S. hominis e S. intermedius. In all the samples of handmade coalho cheese there was a prevalence of S. aureus; while at industrial samples S. xylosus (87.5%) and S. cohnii spp cohnii (50%) were predominant. The presence of the femA gene was detected in 95% (38/40) isolates of positive Staphylococcus coagulase and 16.4% (9/55) of coagulasenegative isolates. Among the enterotoxin encoding genes evaluated, there was prevalence of the seh gene (53.2%) in coagulase-positive strains and of the sec gene (46.8%) in coagulase-negative strains. The results suggest a re-evaluation of the Brazilian microbiological standards in relation to the genus Staphylococcus in foods.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectENVENENAMENTO ALIMENTAR
dc.subjectLACTICÍNIOS
dc.subjectENTEROTOXINAS ESTAFILOCÓCICAS
dc.titleDiversity of Staphylococcus coagulase: positive and negative strains of coalho cheese and detection of enterotoxin encoding genes.
dc.typeArtigo de periódico
dc.subject.thesagroIntoxicação Alimentar
dc.subject.nalthesaurusDairy products
dc.subject.nalthesaurusEnterotoxins
dc.subject.nalthesaurusStaphylococcal intoxication
riaa.ainfo.id1118013
riaa.ainfo.lastupdate2025-05-22
dc.identifier.doihttps://doi.org/10.5380/bceppa.v36i1.57553
dc.contributor.institutionANA PAULA COLARES DE ANDRADE, Universidade Federal do Ceará, Departamento de Engenharia de Alimentos; MARIA DE FÁTIMA BORGES, Embrapa Agroindústria Tropical, Doutora, Pesquisadora aposentada.; EVÂNIA ALTINA TEIXEIRA DE FIGUEIREDO, Universidade Federal do Ceará, Departamento de Engenharia de Alimentos; EDNA FROEDER ARCURI, CNPGL.
Appears in Collections:Artigo em periódico indexado (CNPGL)

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