Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1118709
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dc.contributor.authorFONSECA, J. F. daeng
dc.contributor.authorBATISTA, R. I. T. P.eng
dc.contributor.authorTREVIZAN, J. T.eng
dc.contributor.authorSOUZA-FABJAN, J. M. G.eng
dc.contributor.authorBRANDÃO, F. Z.eng
dc.contributor.authorCAMARGO, L. S. de A.eng
dc.date.accessioned2020-01-14T18:14:47Z-
dc.date.available2020-01-14T18:14:47Z-
dc.date.created2020-01-14
dc.date.issued2019
dc.identifier.citationSemina: Ciências Agrárias, Londrina, v. 40, n. 6, supp. 3, p. 3789-3796, 2019.eng
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1118709-
dc.descriptionAbstract We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student?s t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that transcervical transfer of bovine oocytes to a goat?s uterus could present an alternative to nuclear maturation.eng
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectNonsurgical embryo transfer techniqueeng
dc.subjectEx-situ maturationeng
dc.subjectIn vitro maturationeng
dc.subjectBovine oocyteseng
dc.subjectCOCseng
dc.titleGoat incubator: can bovine oocytes be matured in the uterine horn of a goat?eng
dc.typeArtigo de periódicoeng
dc.date.updated2020-01-14T18:14:47Z
dc.subject.nalthesaurusGoatseng
riaa.ainfo.id1118709eng
riaa.ainfo.lastupdate2020-01-14
dc.identifier.doi10.5433/1679-0359.2019v40n6Supl3p3789eng
dc.contributor.institutionJEFERSON FERREIRA DA FONSECA, CNPC; Ribrio Ivan Tavares Pereira Batista; Juliane Teramachi Trevizan; Joanna Maria Gonçalves Souza-Fabjan; Felipe Zandonadi Brandão; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL.eng
Aparece nas coleções:Artigo em periódico indexado (CNPGL)

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