Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1119144
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dc.contributor.authorAZÊVEDO, H. S. F. da S.eng
dc.contributor.authorRUFINO, P. B.eng
dc.contributor.authorAZEVEDO, J. M. A. deeng
dc.contributor.authorSILVA, L. M. daeng
dc.contributor.authorWADT, L. H. de O.eng
dc.contributor.authorCAMPOS, T. deeng
dc.date.accessioned2020-01-22T00:37:56Z-
dc.date.available2020-01-22T00:37:56Z-
dc.date.created2020-01-21
dc.date.issued2019
dc.identifier.citationBioscience Journal, v. 35, n. 4, p. 1188-1197, jul./ago. 2019.eng
dc.identifier.issn1981-3163eng
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1119144-
dc.descriptionThe objective of this study was to test the efficiency of preservation and maceration methods for Euterpe precatoria leaflet tissue to obtain genomic DNA for molecular studies. The leaflets of E. precatoria were collected in an experimental field at Embrapa Acre, Brazil. The study was conducted in a completely randomized design with 10 replicates, in a 12 × 2 factorial structure, with 12 storage treatments (fresh; lyophiliser 3 days; refrigerator 3, 5, and 7 days; silica gel 7, 10, 20, and 30 days; and transport buffer 3, 5, and 7 days) and two leaf tissue maceration methods (liquid nitrogen and the TissueLyser®). Statistically significant differences in the obtained DNA concentration were found between the maceration and storage treatments. The TissueLyser® macerator produced higher DNA concentrations when compared to liquid nitrogen. For the storage treatments, five groups were formed based on DNA concentration when macerated with the TissueLyser® and two groups when macerated with liquid nitrogen. The DNA concentrations ranged from 285.00 ng/uL (7 days in transport buffer) to 702.00 ng/uL (30 days in silica gel) when the leaflets were macerated with liquid nitrogen, and from 572.73 ng/uL (30 days in silica gel) to 2,850.00 ng/uL (3 days in lyophiliser) using the TissueLyser® macerator. The DNA purity (A260/A280 nm) varied from 1.30 to 1.70 when the leaflets were macerated with liquid nitrogen and from 1.30 to 1.90 with the TissueLyser® macerator. Despite the variations in leaf tissue preservation and DNA concentration, all treatments were effective for DNA isolation and it was possible to amplify genomic regions of microsatellite markers by PCR. It was concluded that leaflets of E. precatoria stored in a lyophiliser and processed with an automatic macerator resulted in satisfactory DNA for molecular studies.eng
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectLeafleteng
dc.subjectHojaseng
dc.subjectExtraccióneng
dc.subjectMaceracióneng
dc.subjectEmbrapa Acreeng
dc.subjectRio Brancoeng
dc.subjectAcreeng
dc.subjectAmazônia Ocidentaleng
dc.subjectWestern Amazoneng
dc.subjectAmazonia Occidentaleng
dc.titlePreservation and maceration of Amazon açai leaflet tissue to obtain genomic DNA.eng
dc.typeArtigo de periódicoeng
dc.date.updated2020-01-27T11:11:11Z
dc.subject.thesagroAçaíeng
dc.subject.thesagroExtraçãoeng
dc.subject.thesagroFolhaeng
dc.subject.thesagroMaceraçãoeng
dc.subject.thesagroCampo Experimentaleng
dc.subject.thesagroDNAeng
dc.subject.nalthesaurusEuterpe precatoriaeng
dc.subject.nalthesaurusLeaveseng
dc.subject.nalthesaurusExtractioneng
dc.subject.nalthesaurusMacerationeng
riaa.ainfo.id1119144eng
riaa.ainfo.lastupdate2020-01-27 -02:00:00
dc.identifier.doi10.14393/BJ-v35n4a2019-42190eng
dc.contributor.institutionHellen Sandra Freires da Silva Azêvedo, Fundação Oswaldo Cruz (FIOCRUZ), Rede Bionorte; Polinar Bandeira Rufino, Faculdade Meta (FAMETA); José Marlo Araújo de Azevedo, Instituto Federal do Acre (IFAC); LUCIELIO MANOEL DA SILVA, CPAF-AC; LUCIA HELENA DE OLIVEIRA WADT, CPAF-RO; TATIANA DE CAMPOS, CPAF-AC.eng
Aparece nas coleções:Artigo em periódico indexado (CPAF-AC)

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