Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1120636
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dc.contributor.authorAGOSTINETTO, D.eng
dc.contributor.authorBENEMANN, D. P.eng
dc.contributor.authorCECHIN, J.eng
dc.contributor.authorNOHATTO, M. A.eng
dc.contributor.authorLANGARO, A. C.eng
dc.contributor.authorPIASECKI, C.eng
dc.contributor.authorVARGAS, L.eng
dc.date.accessioned2020-02-28T18:10:54Z-
dc.date.available2020-02-28T18:10:54Z-
dc.date.created2020-02-28
dc.date.issued2019
dc.identifier.citationAgronomy JOurnal, v. 3, n. 3, p. 1239-1246, March 2019.eng
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1120636-
dc.descriptionHerbicides are stressors that can have negative effects on plants. In Oryza sativa (L.), differential gene expression may be evalu-ated through real-time reverse transcription quantitative poly-merase chain reaction (RT-qPCR). The aim of this study was to evaluate the stability of candidate reference genes and to quan-tify the relative expression of oxidative stress genes at different times (12, 24, 48, and 96 hours after treatment [HAT]) with penoxsulam, cyhalofop-butyl, and bentazon herbicides. Norm-Finder, BestKeeper, and GeNorm software and the compara-tive ∆Ct method were used to assess expression stability and to determine the RT-qPCR threshold values of the candidate reference genes. The UBQ5 gene was the most stable among the reference genes analyzed. The gene expression results showed upregulation of OsCAT and OsMnSOD1 genes at all times after herbicide application. The OsA PX 2 and OsGST3 genes showed increased gene expression at 12 and 96 HAT for all herbicides. The OsHO -1 gene had the most significant expression changes, with maximum expression levels at 24 HAT with bentazon and at 96 HAT with penoxsulam and cyhalofop-butyl. Overall, antioxidant system gene expression increased after the applica-tion of bentazon, penoxsulam, and cyhalofop-butyl in riceeng
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleGene expression related to oxidative stress induced by herbicides in rice.eng
dc.typeArtigo de periódicoeng
dc.date.updated2020-02-28T18:10:54Z
dc.subject.thesagroOryza Sativaeng
dc.subject.nalthesaurusRiceeng
dc.subject.nalthesaurusGene expressioneng
dc.subject.nalthesaurusOxidative stresseng
dc.subject.nalthesaurusHerbicideseng
riaa.ainfo.id1120636eng
riaa.ainfo.lastupdate2020-02-28
dc.identifier.doi10.2134/agronj2018.07.0448eng
dc.contributor.institutionDirceu Agostinetto, D.P. Benemann, J. Cechin, C. Piasecki, Dep. of Crop Protection, Federal Univ. of Pelotas- Campus Capão do Leão, Zip Code 96010-900, Capão do Leão, RS– Brazileng
dc.contributor.institutionDaiane P. Benemann, Dep. of Crop Protection, Federal Univ. of Pelotas- Campus Capão do Leão, Zip Code 96010-900, Capão do Leão, RS– Brazileng
dc.contributor.institutionJoanei Cechin, Dep. of Crop Protection, Federal Univ. of Pelotas- Campus Capão do Leão, Zip Code 96010-900, Capão do Leão, RS– Brazieng
dc.contributor.institutionMarcos A. Nohatto, Dep. of Agriculture, Federal Institute Catarinense-Campus Santa Rosa do Sul, Santa Rosa do Sul, SC– Brazileng
dc.contributor.institutionAna C. Langaro, Dep. of Agricultural and Environmental Engineering, Federal Rural Univ. of Rio de Janeiro, Seropédica, RJ- Brazileng
dc.contributor.institutionCristiano Piasecki, Dep. of Crop Protection, Federal Univ. of Pelotas- Campus Capão do Leão, Zip Code 96010-900, Capão do Leão, RS– Brazileng
dc.contributor.institutionLEANDRO VARGAS, CNPT.eng
Appears in Collections:Artigo em periódico indexado (CNPT)

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