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dc.contributor.authorGIGLIOTI, R.eng
dc.contributor.authorGUTMANIS, G.eng
dc.contributor.authorKATIKI, L. M.eng
dc.contributor.authorOKINO, C. H.eng
dc.contributor.authorOLIVEIRA, M. C. de S.eng
dc.contributor.authorVERCESI FILHO, A. E.eng
dc.date.accessioned2020-04-03T00:39:33Z-
dc.date.available2020-04-03T00:39:33Z-
dc.date.created2020-03-10
dc.date.issued2020
dc.identifier.citationFood Chemistry, v. 313, 2020. 126167.eng
dc.identifier.issn0308-8146eng
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1121088-
dc.descriptionCows milk may contain two types of - casein: A1 and A2. A1 digestion is associated with the release of - casomorphine-7 peptide, which can cause adverse gastrointestinal effects. Two methods high-resolution melting (HRM) and rhAmp® SNP genotyping - were developed to identify the - casein gene (CSN2) A1 and A2 alleles directly in milk. DNA milk samples from 45 animals were examined and 10 samples were also sequenced to confirm the accuracy of the assays. The analytical sensitivities of both strategies for A1 allele identification were evaluated by testing decreasing dilutions of A1 allele DNA copies (500 - 5 copies) in the A2 sample. The limits of detection for A1 in A2 samples were 10% (100 copies) and 2% (10 copies) for HRM and rhAmp, respectively. Both techniques were specific, differentiating between A1 and A2 alleles. However, we recommend rhAmp genotyping testing over HRM because of its enhanced sensitivity for A1.eng
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectB caseineng
dc.subjectHRMeng
dc.subjectRhAmpeng
dc.titleNew high-sensitive rhAmp method for A1 allele detection in A2 milk samples.eng
dc.typeArtigo de periódicoeng
dc.date.updated2020-04-20T11:11:11Z
dc.subject.nalthesaurusGenotypingeng
dc.subject.nalthesaurusAlleleseng
dc.format.extent27 p.eng
riaa.ainfo.id1121088eng
riaa.ainfo.lastupdate2020-04-20 -03:00:00
dc.identifier.doihttps://doi.org/10.1016/j.foodchem.2020.126167eng
dc.contributor.institutionRodrigo Giglioti, Instituto de Zootecnia; Gunta Gutmanis, Instituto de Zootecnia; Luciana Morita Katiki, Instituto de Zootecnia; CINTIA HIROMI OKINO, CPPSE; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE; Anibal Eugênio Vercesi Filho, Instituto de Zootecnia.eng
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