Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1123250
Título: Auxin independent1 (Axi1) as an endogenous gene for copy number determination in transgenic tobacco.
Autoria: LOPES, S. da S.
COSTA, J. F. da V.
BARROS, B. de A.
CARNEIRO, A. A.
SOUSA, S. M. de
Afiliação: Simara da Silva Lopes, Universidade Federal de São João del-Rei; Jéssica Fabiane da Veiga Costa, Centro Universitário de Sete Lagoas; BEATRIZ DE ALMEIDA BARROS, CNPMS; ANDREA ALMEIDA CARNEIRO, CNPMS; SYLVIA MORAIS DE SOUSA TINOCO, CNPMS.
Ano de publicação: 2020
Referência: Crop Breeding and Applied Biotechnology, v. 20, n. 2, e289220212, 2020.
Conteúdo: Determining the copy number is important because it can greatly influence the expression level and genetic stability of transgenes. This study aimed to establish a methodology that can estimate the transgene copy number in tobacco (Nicotiana tabacum) using the Auxin independent1 (Axi1) gene as an endogenous control. Tobacco c.v. Petit Havana plants were transformed via Agrobacterium tumefaciens with a pMCG1005 vector that contained Bar as a selective gene. Oligonucleotide efficiency was determined and a qPCR using the 2-ΔCt and 2-ΔΔCt methods was performed. Bar was the target gene and Axi1 was the endogenous control in five transgenic tobacco events. The results showed that the Axi1 gene was donated by the maternal parent N. sylvestris when interspecific hybridization occurred between N. sylvestris and N. tomentosiformis. The copy number results agreed with the segregation ratios for the Bar gene in T1 plants, which confirmed that Axi1 is a single copy gene that can be used as an endogenous control.
Thesagro: Planta Transgênica
Gene
Nicotiana Tabacum
Palavras-chave: Cópia transgênica
Tabaco
Controle endógeno
Digital Object Identifier: http://dx.doi.org/10.1590/1984- 70332020v20n2a28
Tipo do material: Artigo de periódico
Acesso: openAccess
Aparece nas coleções:Artigo em periódico indexado (CNPMS)

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