Título:	Stability and tissue-specific Cry10Aa overexpression improves cotton resistance to the cotton boll weevil.
Autoria:	RIBEIRO, T. P. BASSO, M. F. CARVALHO, M. H. de MACEDO, L. L. P. de SILVA, D. M. L. da LOURENCO-TESSUTTI, I. T. OLIVEIRA-NETO, O. B. de CAMPOS-PINTO, E. R. de LUCENA, W. A. SILVA, M. C. M. da TRIPODE, B. M. D. ABREU-JARDIM, T. P. F. MIRANDA, J. E. ALVES-FERREIRA, M. MORGANTE, C. V. GROSSI-DE-SA, M. F.
Afiliação:	THUANNE PIRES RIBEIRO, UNB; MARCOS FERNANDO BASSO; MAYARA HOLANDA DE CARVALHO; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; DAGNA MARIA LAURINDO DA SILVA; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; OSMUNDO BRILANTE DE OLIVEIRA-NETO; EDUARDO ROMANO DE CAMPOS PINTO, Cenargen; WAGNER ALEXANDRE LUCENA, Cenargen; MARIA CRISTINA MATTAR DA SILVA, Cenargen; BRUNA MENDES DINIZ TRIPODE, CNPA; TATIANNE PIZA FERRARI ABREU-JARDIM; JOSE EDNILSON MIRANDA, CNPA; MARCIO ALVES-FERREIRA, UFRJ; CAROLINA VIANNA MORGANTE, CPATSA; MARIA FATIMA GROSSI DE SA, Cenargen.
Ano de publicação:	2020
Referência:	Biotechnology Research and Innovation, v. 3, p. 27-41, 2020.
Conteúdo:	The cotton boll weevil (CBW, Anthonomus grandis) is the most destructive cotton insect pest affecting cotton crops. To overcome this problem, CBW-resistant genetically modified cotton plants overexpressing Bacillus thuringiensis entomotoxins were successfully obtained. Previous results showed that the overexpression of Cry10Aa protoxin led to high mortality of the CBW larvae in greenhouse conditions. In this study, we advanced three more generations (T2 to T4), with several cotton events constitutively overexpressing the Cry10Aa protoxin, and the transgene stability and agronomic performance were investigated. In addition, stable transgenic cotton overexpressing the Cry10Aa active (Cry10Aa protoxin lacking the -helix N-terminal) driven by cotton flower bud-specific promoters were generated and characterized. Cotton events constitutively or tissue-specifically overexpressing the Cry10Aa protein (protoxin or active) represented mortality percentages of the CBW larva of up to 85 % in plants under greenhouse conditions. Events overexpressing the Cry10Aa active under control of the flower bud-specific promoter showed higher protein accumulation in stamens and carpels compared to the events with constitutive expression. Our findings suggested that the high stability of the Cry10Aa transgene and the elevated expression level and protein accumulation in flower bud tissues (primarily in stamen and carpels) contribute to improved resistance to CBW larvae. Finally, some notable events were selected with potential for future field trials in different cotton- producing regions of Brazil. Therefore, cotton events overexpressing high levels of the Cry10Aa protein in flower bud tissue may have a strong potential for commercial use in the integrated management of CBW.
Thesagro:	Algodão Inseto Praga Praga de Planta
NAL Thesaurus:	Insect pests Resistance management Cotton
Palavras-chave:	Praga de inseto Resistência gestão Bacillus Bicudo do algodão Herança estável
Digital Object Identifier:	https://doi.org/10.1016/j.biori.2019.12.003
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CPATSA)