Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1126471
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dc.contributor.authorCARRIERO, M. M.
dc.contributor.authorHENRIQUE SILVA, F.
dc.contributor.authorMEIRA, C. M.
dc.contributor.authorGATO, I. M. Q.
dc.contributor.authorCAETANO, A. R.
dc.contributor.authorLOBO, F. P.
dc.contributor.authorALVES, A. L.
dc.contributor.authorVARELA, E. S.
dc.contributor.authorMAIA, A. A. M.
dc.date.accessioned2021-01-23T09:08:33Z-
dc.date.available2021-01-23T09:08:33Z-
dc.date.created2020-11-11
dc.date.issued2020
dc.identifier.citationGenetics and Molecular Biology, v. 43, n. 4, e20200006, 2020.
dc.identifier.issn1678-4685
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1126471-
dc.descriptionIn the present study, the complete characterization of cDNA and genomic sequences of IL-1B and IL-8, as well as the expression profile of these genes in the South American fish pacu (Piaractus mesopotamicus) is provided. The full-length pmIL-1B cDNA was composed of 1208 nucleotides that would produce a precursor peptide with 273 amino acid residues. A putative caspase-1 cleavage site, similar to what is found in mammalian IL-1B, was identified producing a mature peptide with a theoretical molecular weight of 17.21 kDa. The pmIL-8 cDNA sequence consisted of 1019 nucleotides which encoded a 95-amino acid protein with a theoretical molecular weight of 10.43 kDa that showed all typical CXC chemokine features, including a 20-residue signal peptide and four conserved cysteine residues. Constitutive mRNA expression was detected for both genes in the liver, head kidney, gill, intestine, skin and spleen. After a bacterial challenge, up-regulation was detected for both pmIL-1B and pmIL-8 in the spleen and head kidney at 12 h post-infection. At 24 h post-infection there was a decrease in the expression of both genes, with pmIL-8 showing a significant down-regulation in the liver and head kidney when compared to the control groups.
dc.language.isoeng
dc.rightsopenAccesseng
dc.subjectBacterial infection
dc.subjectFish immunology
dc.subjectSouth American fish
dc.titleMolecular characterization and gene expression analysis of the pro-inflammatory cytokines IL-1beta and IL-8 in the South American fish Piaractus mesopotamicus challenged with Aeromonas dhakensis.
dc.typeArtigo de periódico
dc.subject.thesagroPeixe
dc.subject.thesagroPacu
dc.subject.thesagroInfecção
dc.subject.nalthesaurusGene expression
dc.subject.nalthesaurusPiaractus mesopotamicus
dc.subject.nalthesaurusFish
dc.subject.nalthesaurusInterleukins
riaa.ainfo.id1126471
riaa.ainfo.lastupdate2021-01-22
dc.identifier.doihttps://doi.org/10.1590/1678-4685-gmb-2020-0006
dc.contributor.institutionMATEUS MALDONADO CARRIERO, USP; FLAVIO HENRIQUE SILVA, UFSCAR; CAROLINE MUNHOZ MEIRA, USP; IGOR MATEUS QUEIROZ GATO, USP; ALEXANDRE RODRIGUES CAETANO, Cenargen; FRANCISCO PEREIRA LOBO; ANDERSON LUIS ALVES, SIN; EDUARDO SOUSA VARELA, CNPASA; ANTONIO AUGUSTO MENDES MAIA, USP.
Appears in Collections:Artigo em periódico indexado (CNPASA)

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