Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1127111
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Campo DCValorIdioma
dc.contributor.authorSANTOS, M. R. A. dos
dc.contributor.authorPAZ, E. S.
dc.date.accessioned2020-11-27T09:04:16Z-
dc.date.available2020-11-27T09:04:16Z-
dc.date.created2020-11-26
dc.date.issued2020
dc.identifier.citationInternational Journal of Development Research, v. 10, n. 11, p. 41853-41856, Nov. 2020.
dc.identifier.issn2230-9926
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1127111-
dc.descriptionIn vitro cell suspension cultivation systems have been largely reported as safe and standardized methods of production of secondary metabolites with medicinal and agricultural interest. Capsicum frutescensis one of the most widely grown vegetable in the world and its biological activities have been demonstrated against insects, fungi, bacteria and other groups of organisms. The objective of this study was to establish a protocol for dedifferentiation of leaf cells of the cultivar C. frutescens cv. Stromboli and to determine the growth pattern of the calluses with a focus on the deceleration phase, when the callus cells must be subcultured into a liquid medium in order to establish cell suspension cultivations aiming at the production of secondary metabolites. The explants were inoculated into a medium supplemented with BA and 2,4-D in factorial combinations. The percentage of callus induction (%CI), the explant area covered by callus cells (ACCC) and the fresh weight of the calluses were evaluated. The procedures that resulted in higher proliferation of callus cells were repeated in order to determine the growth curve of the calluses. The treatment that resulted in the highest %CI, ACCC and callus weight was 1.5 mg L-1 2,4-D. The calluses produced were friable and whitish and their growth pattern followed a sigmoid shape. The deceleration phase started on the 39th day of cultivation.
dc.language.isoeng
dc.rightsopenAccesseng
dc.subjectCapsicum frutescens cv Stromboli
dc.subjectCélula foliar
dc.subjectMetabólicos secundários
dc.subjectProtocolo de diferenciação celular
dc.subjectLeaf cells
dc.subjectCallogenesis
dc.subjectGrowth curves
dc.subjectDeceleration phase
dc.subjectFase de desaceleração
dc.subjectEmbrapa Rondônia
dc.subjectPorto Velho (RO)
dc.subjectRondônia
dc.subjectAmazônia Ocidental
dc.subjectWestern Amazon
dc.titleDedifferentation of leaf cells and growth pattern of caluses of Capsicum frutescens cv. Stromboli.
dc.typeArtigo de periódico
dc.subject.thesagroCultura In Vitro
dc.subject.thesagroCalogênese
dc.subject.thesagroCurva de Crescimento
dc.subject.nalthesaurusIn vitro culture
dc.subject.nalthesaurusCallus formation
dc.subject.nalthesaurusSecondary metabolites
dc.subject.nalthesaurusCell dedifferentiation
riaa.ainfo.id1127111
riaa.ainfo.lastupdate2020-11-26
dc.identifier.doi10.37118/ijdr.20362.11.2020
dc.contributor.institutionMAURICIO REGINALDO ALVES DOS SANTOS, CPAF-RO; Universidade Federal de Rondonia.
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