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dc.contributor.authorARAÚJO, J. F.
dc.contributor.authorANDRIOLI, A.
dc.contributor.authorPINHEIRO, R. R.
dc.contributor.authorSIDER, L. H.
dc.contributor.authorSOUSA, A. L. M. de
dc.contributor.authorAZEVEDO, D. A. A. de
dc.contributor.authorPEIXOTO, R. M.
dc.contributor.authorLIMA, A. M. C.
dc.contributor.authorDAMASCENO, E. M.
dc.contributor.authorSOUZA, S. C. R.
dc.contributor.authorTEIXEIRA, M. F. da S.
dc.date.accessioned2020-11-30T09:01:36Z-
dc.date.available2020-11-30T09:01:36Z-
dc.date.created2020-11-29
dc.date.issued2020
dc.identifier.citationPLoSONE, v. 15, n. 11, e0239916, Nov. 2020.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1127175-
dc.descriptionAbstract: This study aimed to evaluate by means of Nested Polymerase Chain Reaction (nPCR), co-cultivation and sequencing, with genetic comparison between strains (mother/newborn), the occurrence of vertical transmission of Small Ruminant Lentiviruses (SRLV) from naturally occurring nannies infected for their offspring. For the detection of SRLV seropositive progenitors, blood was collected from 42 nannies in the final third of gestation in tubes with and without anticoagulant. The diagnostic tests used were Western Blot (WB) and nPCR. During the period of birth, the same blood collection procedure was performed on 73 newborns at zero hours of birth, with the same diagnostic tests. Seventeen blood samples from seven-day-old kids, proven positive for SRLV by nPCR, chosen at random, were subjected to coculture in goat synovial membrane (GSM) cells for 105 days. The pro-viral DNA extracted from the cell supernatant from the coculture was subjected to nPCR. For DNA sequencing from the nPCR products, nine positive samples were chosen at random, four nannies with their respective offspring, also positive. Each sample was performed in triplicate, thus generating 27 nPCR products of which only 19 were suitable for analysis. Among the 42 pregnant goats, in 50% (21/42) pro-viral DNA was detected by nPCR, while in the WB, only 7.14% (3/42) presented antibodies against SRLV. Regarding neonates, of the 73 kids, 34 (46.57%) were positive for the virus, using the nPCR technique, while in the serological test (WB), three positive animals (4.10%) were observed. The coculture of the 17 samples with a positive result in the nPCR was confirmed in viral isolation by amplification of the SRLV pro-viral DNA. When aligned, the pro-viral DNA sequences (nannies and their respective offspring) presented homology in relation to the standard strain CAEV Co. It was concluded that the transmission of SRLV through intrauterine route was potentially the source of infection in the newborn goats.
dc.language.isoeng
dc.rightsopenAccesseng
dc.subjectSRLV
dc.titleVertical transmissibility of small ruminant lentivirus.
dc.typeArtigo de periódico
dc.subject.nalthesaurusGenetic techniques and protocols
dc.subject.nalthesaurusLentivirus
dc.subject.nalthesaurusSheep diseases
dc.subject.nalthesaurusGoat diseases
dc.subject.nalthesaurusGoats
riaa.ainfo.id1127175
riaa.ainfo.lastupdate2020-11-29
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0239916
dc.contributor.institutionJUSCILÂNIA FURTADO ARAÚJO; ALICE ANDRIOLI, CNPC; RAYMUNDO RIZALDO PINHEIRO, CNPC; LUCIA HELENA SIDER, CNPC; ANA LÍDIA MADEIRA DE SOUSA; DALVA ALANA ARAGÃO DE AZEVEDO; RENATO MESQUITA PEIXOTO; ANA MILENA CESAR LIMA; EDGAR MARQUES DAMASCENO; SAMARA CRISTINA ROCHA SOUZA; MARIA FÁTIMA DA SILVA TEIXEIRA.
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