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dc.contributor.authorNERY, F. M. B.
dc.contributor.authorMELO, F. L.
dc.contributor.authorBOITEUX, L. S.
dc.contributor.authorRIBEIRO, S. da G.
dc.contributor.authorRESENDE, R. O.
dc.contributor.authorORÍLIO, A. F.
dc.contributor.authorBATISTA, J. G.
dc.contributor.authorLIMA, M. F.
dc.contributor.authorPEREIRA-CARVALHO, R. C.
dc.date.accessioned2020-12-17T09:03:36Z-
dc.date.available2020-12-17T09:03:36Z-
dc.date.created2020-12-16
dc.date.issued2020
dc.identifier.citationViruses, v. 12, n. 9, 950, 2020
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1128264-
dc.descriptionIn a systematic field survey for plant-infecting viruses, leaf tissues were collected from trees showing virus-like symptoms in Brazil. After viral enrichment, total RNA was extracted and sequenced using the MiSeq platform (Illumina). Two nearly full-length picorna-like genomes of 9534 and 8158 nucleotides were found associated with Hovenia dulcis (Rhamnaceae family). Based upon their genomic information, specific primers were synthetized and used in RT-PCR assays to identify plants hosting the viral sequences. The larger contig was tentatively named as Hovenia dulcis-associated virus 1 (HDaV1), and it exhibited low nucleotide and amino acid identities with Picornavirales species. The smaller contig was related to insect-associated members of the Dicistroviridae family but exhibited a distinct genome organization with three non-overlapping open reading frames (ORFs), and it was tentatively named as Hovenia dulcis-associated virus 2 (HDaV2). Phylogenetic analysis using the amino acid sequence of RNA-dependent RNA polymerase (RdRp) revealed that HDaV1 and HDaV2 clustered in distinct groups, and both viruses were tentatively assigned as new members of the order Picornavirales. HDaV2 was assigned as a novel species in the Dicistroviridae family. The 50 ends of both viruses are incomplete. In addition, a nucleotide composition analysis (NCA) revealed that HDaV1 and HDaV2 have similarities with invertebrate-infecting viruses, suggesting that the primary host(s) of these novel virus species remains to be discovered.
dc.language.isoeng
dc.rightsopenAccesseng
dc.subjectHDaV1
dc.subjectHDaV2
dc.titleMolecular characterization of Hovenia Dulcis-associated virus 1 (HDaV1) and 2 (HDaV2): new tentative species within the Order Picornavirales.
dc.typeArtigo de periódico
dc.subject.thesagroHovenia Dulcis
dc.subject.nalthesaurusViruses
dc.subject.nalthesaurusMetagenomics
dc.subject.nalthesaurusPicornavirales
riaa.ainfo.id1128264
riaa.ainfo.lastupdate2020-12-16
dc.identifier.doihttps://doi.org/10.3390/v12090950
dc.contributor.institutionFLÁVIA M. B. NERY, UNIVERSIDADE DE BRASÍLIA; FERNANDO L. MELO, UNIVERSIDADE DE BRASÍLIA; LEONARDO SILVA BOITEUX, CNPH; SIMONE DA GRACA RIBEIRO, Cenargen; RENATO O. RESENDE, UNIVERSIDADE DE BRASÍLIA; ANELISE F. ORÍLIO, UNIVERSIDADE DE BRASÍLIA; JOSIANE G. BATISTA, UNIVERSIDADE DE BRASÍLIA; MIRTES FREITAS LIMA, CNPH; RITA C. PEREIRA-CARVALHO, UNIVERSIDADE DE BRASÍLIA.
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