Título:	Actinobacteria from Antarctica as a source for anticancer discovery.
Autoria:	SILVA, L. J. da CREVELIN, E. J. SOUZA, D. T. LACERDA JÚNIOR, G. V. OLIVEIRA, V. M. RUIZ, A. L. T. G. ROSA, L. H. MORAES, L. A. B. MELO, I. S. de
Afiliação:	LEONARDO JOSÉ SILVA, ESALQ-USP; EDUARDO JOSÉ CREVELIN, FFCLRP-USP; DANILO TOSTA SOUZA, FFCLRP-USP; GILENO VIEIRA LACERDA JÚNIOR; VALERIA MAIA DE OLIVEIRA, CPQBA-UNICAMP; ANA LUCIA TASCA GOIS RUIZ, FCF-UNICAMP; LUIZ HENRIQUE ROSA, UFMG; LUIZ ALBERTO BERALDO MORAES, FFCLRP-USP; ITAMAR SOARES DE MELO, CNPMA.
Ano de publicação:	2020
Referência:	Scientific Reports, v. 10, article 13870, 2020.
Páginas:	p. 1-15.
Conteúdo:	Abstract: Although many advances have been achieved to treat aggressive tumours, cancer remains a leading cause of death and a public health problem worldwide. Among the main approaches for the discovery of new bioactive agents, the prospect of microbial secondary metabolites represents an effective source for the development of drug leads. In this study, we investigated the actinobacterial diversity associated with an endemic Antarctic species, Deschampsia antarctica, by integrated culture-dependent and culture-independent methods and acknowledged this niche as a reservoir of bioactive strains for the production of antitumour compounds. The 16S rRNA-based analysis showed the predominance of the Actinomycetales order, a well-known group of bioactive metabolite producers belonging to the Actinobacteria phylum. Cultivation techniques were applied, and 72 psychrotolerant Actinobacteria strains belonging to the genera Actinoplanes, Arthrobacter, Kribbella, Mycobacterium, Nocardia, Pilimelia, Pseudarthrobacter, Rhodococcus, Streptacidiphilus, Streptomyces and Tsukamurella were identified. The secondary metabolites were screened, and 17 isolates were identified as promising antitumour compound producers. However, the bio-guided assay showed a pronounced antiproliferative activity for the crude extracts of Streptomyces sp. CMAA 1527 and Streptomyces sp. CMAA 1653. The TGI and LC50 values revealed the potential of these natural products to control the proliferation of breast (MCF-7), glioblastoma (U251), lung/non-small (NCI-H460) and kidney (786-0) human cancer cell lines. Cinerubin B and actinomycin V were the predominant compounds identified in Streptomyces sp. CMAA 1527 and Streptomyces sp. CMAA 1653, respectively. Our results suggest that the rhizosphere of D. antarctica represents a prominent reservoir of bioactive actinobacteria strains and reveals it as an important environment for potential antitumour agents.
Thesagro:	Bactéria não Patogênica Câncer
NAL Thesaurus:	Actinobacteria Antarctica
Palavras-chave:	Anticancer activity Antitumoral activity
ISSN:	2045-2322
Digital Object Identifier:	https://doi.org/10.1038/s41598-020-69786-2
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPMA)