Título:	Genetic variability of Colletotrichum sublineolum through ISSR markers.
Autoria:	GUIMARÃES, T. S. BARELLI, M. A. A. CORREA, C. L. SILVA, V. P. da FLORIANO, A. J. S. SANDER, N. L. DUARTE, A. V. M. ZAGO, B. W. RIBEIRO, J. L. B. TARDIN, F. D. SILVA, D. D. da
Afiliação:	Thallita Santos Guimarães, Universidade do Estado de Mato Grosso; Marco Antonio Aparecido Barelli, Universidade do Estado de Mato Grosso; Carla Lima Corrêa, Universidade do Estado de Mato Grosso; Valvenarg Pereira da Silva, Universidae do Estado de Mato Grosso; Alex Junior Sandol Floriano, Universidade do Estado de Mato Grosso; Nilo Leal Sander, Universidade do Estado de Mato Grosso; Aline Vidor Melão Duarte, Universidade do Estado de Mato Grosso; Bruno Wagner Zago, Universidade do Estado de Mato Grosso; Jackson Lauro Borges Ribeiro, Universidade do Estado de Mato Grosso; FLAVIO DESSAUNE TARDIN, CNPMS; DAGMA DIONISIA DA SILVA, CNPMS.
Ano de publicação:	2021
Referência:	Research, Society and Development, v. 10, n. 2, e20210212223, 2021.
Conteúdo:	The occurrence of diseases is a limiting factor in the development of sorghum crop. Among the diseases that causes losses in sorghum production, anthracnose is the main and most severe, mainly by the genetic variability of the pathogen. In this context, the aim of this study was to evaluate the genetic variability of Colletotrichum sublineolum isolates. DNA were extracted from 56 monosporic isolates of C. sublineolum using a DNA extraction kit, and to perform the analysis of genetic diversity of the isolates were used ISSR primers. After amplification, it was determined the polymorphic information content (PIC), allelic frequency, UPGMA and Tocher clustering analyzes and, using software Structure, the genetic structure. According to the descriptive analysis of the genetic variability of C. Sublineolum isolates, primer AP1 presented the higher value of polymorphic information content (PIC). The higher allelic frequency was observed in loci 06, 09, 10, and 24, and the lowest in locus 02. As for the clustering method, it was observed a tendency of grouping C. sublineolum isolates according the geographic origin and, in addition to demonstrating the genetic variability between the C. sublineolum isolates, it was observed the occurrence of introgression among the isolates.
Thesagro:	Sorghum Bicolor Antracnose Marcador Molecular Doença de Planta
Palavras-chave:	Variabilidade genética
Digital Object Identifier:	http://dx.doi.org/10.33448/rsd-v10i2.12223
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPMS)