Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1131989
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dc.contributor.authorPRADO, G. S.
dc.contributor.authorPINHEIRO, T. T.
dc.contributor.authorFARIA, J. C. de
dc.contributor.authorVIANELLO, R. P.
dc.date.accessioned2021-05-25T02:23:10Z-
dc.date.available2021-05-25T02:23:10Z-
dc.date.created2021-05-24
dc.date.issued2021
dc.identifier.citationIn: MOLINARI, H. B. C.; VIEIRA, L. R.; SILVA, N. V. e; PRADO, G. S.; LOPES FILHO, J. F. (Ed.). CRISPR technology in plant genome editing: biotechnology applied to agriculture. Brasília, DF : Embrapa, 2021.
dc.identifier.isbn978-65-87380-10-0
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1131989-
dc.descriptionWe will focus here on the use of the CRISPR/Cas system to induce mutations via NHEJ. The NHEJ mechanism acts in most of the cell cycle and is not based on homology to repair DNA, causing mutations such as insertions or deletions (indels) or even substitutions. In this way, the expression of the genes in the place where the repair occurred by NHEJ can, in general, be interrupted, and the encoded proteins may not be functional. To clarify the process of genome editing by CRISPR, this chapter aims to address the details of the NHEJ mechanism, as well as the use of ribonucleoprotein systems (RNPs) to promote editing, in addition to providing a practical procedure for the design of experiments.
dc.formatil.
dc.language.isoeng
dc.rightsopenAccesseng
dc.subjectCRISPR
dc.subjectCRISPRevolution
dc.subjectNon-homologous
dc.titleGenome editing via non-homologous end-joining (NHEJ) and ribonucleoproteins (RNP).
dc.title.alternativeTecnologia CRISPR na edição genômica de plantas: biotecnologia aplicada à agricultura, 2020.
dc.typeParte de livro
dc.subject.thesagroBiotecnologia
dc.subject.thesagroDNA
dc.subject.thesagroRNA
dc.subject.thesagroEngenharia Genética
dc.subject.thesagroGenoma
dc.subject.nalthesaurusBiotechnology
dc.subject.nalthesaurusGenetic engineering
dc.subject.nalthesaurusNucleases
dc.subject.nalthesaurusPlant genetics
dc.subject.nalthesaurusRibonucleoproteins
dc.subject.nalthesaurusRNA editing
dc.format.extent2p. 47-88.
riaa.ainfo.id1131989
riaa.ainfo.lastupdate2021-05-24
dc.contributor.institutionGUILHERME SOUZA PRADO, especialista visitante CNPAF; THAÍSA TESSUTTI PINHEIRO, especialista visitante CNPAF; JOSIAS CORREA DE FARIA, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF.
Appears in Collections:Capítulo em livro científico (CNPAF)

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