Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1134749
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dc.contributor.authorPIRES, D. R.
dc.contributor.authorMORAIS, A. C. N.
dc.contributor.authorCUNHA, N. C.
dc.contributor.authorMACHADO, L. S.
dc.contributor.authorBARBOSA, L. F. C.
dc.contributor.authorMENDONÇA, J. F. M.
dc.contributor.authorBALARO, M. F. A.
dc.contributor.authorSANTOS, J. P. C.
dc.contributor.authorSOUZA, G. N. de
dc.contributor.authorBARRETO, M. L.
dc.contributor.authorNASCIMENTO, E. R.
dc.date.accessioned2021-09-24T12:01:03Z-
dc.date.available2021-09-24T12:01:03Z-
dc.date.created2021-09-24
dc.date.issued2021
dc.identifier.citationArquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 73, n. 2, p. 293-301, 2021.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1134749-
dc.descriptionMycoplasma bovis is a highly contagious agent associated with several pathologies in cattle. The detection of reactive antibodies to M. bovis by Indirect Enzyme-Linked Immunosorbent Assay (iELISA) identifies if there was an exposure to the microorganism. The current study aimed to optimize an iELISA from M. bovis total cell antigen, applying it to bovine serum samples, and to evaluate risk factors. Serum samples were obtained from 400 cows from 17 herds from Southeast Brazil. In the optimization of iELISA, the following was established: 2 ?g/mL of antigen, sera dilution 1:300, and conjugate dilution 1:15000. The frequency was 62.3% (249/400) of reactive animals and 100% (17/17) of reactive herds. Risk factors were: herds with more than 100 animals (OR= 3.1; CI= 95%); Holstein breed (OR= 72.5; CI= 95%); cows (OR= 29.7; CI= 95%); intensive breeding system (OR= 3.3; CI= 95%); associated small ruminant production (OR= 4.4; CI= 95%); milk production above 500L (OR= 2.9; CI= 95%); no quarantine (OR= 1.5; CI= 95%); mechanical milking (OR= 5.5; CI= 95%) and cases of mastitis (OR=5.5; CI= 95%). The proposed iELISA was able to detect antibodies reactive to M. bovis in bovine serum. Knowledge of these risk factors can assist in the implementation of prophylactic measures.
dc.language.isoeng
dc.rightsopenAccesseng
dc.subjectMastite
dc.subjectHolstein breed
dc.subjectRaça Holandesa
dc.titleProposal of an iELISA for Mycoplasma bovis diagnosis in dairy cattle and associated risk factors.
dc.typeArtigo de periódico
dc.subject.thesagroBovino
dc.subject.thesagroVaca
dc.subject.thesagroMicoplasmose
dc.subject.nalthesaurusMycoplasmosis
dc.subject.nalthesaurusMastitis
dc.subject.nalthesaurusSerology
dc.subject.nalthesaurusCows
riaa.ainfo.id1134749
riaa.ainfo.lastupdate2021-09-24
dc.contributor.institutionUniversidade Federal Fluminense; Universidade Federal Fluminense; Universidade Federal Fluminense; Universidade Federal Fluminense; Universidade Federal Fluminense; Universidade Federal Fluminense; Universidade Federal Fluminense; Fundação Oswaldo Cruz; GUILHERME NUNES DE SOUZA, CNPGL; Universidade Federal Fluminense; Universidade Federal Fluminense.
Appears in Collections:Artigo em periódico indexado (CNPGL)

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