Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1135377
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dc.contributor.authorHUL, L. M.
dc.contributor.authorIBELLI, A. M. G.
dc.contributor.authorSAVOLDI, I. R.
dc.contributor.authorMARCELINO, D. E. P.
dc.contributor.authorFERNANDES, L. T.
dc.contributor.authorPEIXOTO, J. O.
dc.contributor.authorCANTAO, M. E.
dc.contributor.authorHIGA, R. H.
dc.contributor.authorGIACHETTO, P. F.
dc.contributor.authorCOUTINHO, L. L.
dc.contributor.authorLEDUR, M. C.
dc.date.accessioned2021-10-15T18:00:42Z-
dc.date.available2021-10-15T18:00:42Z-
dc.date.created2021-10-15
dc.date.issued2021
dc.identifier.citationScientific Reports, v. 11, n. 1, p. 1-13. 2021.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1135377-
dc.descriptionLocomotor problems are among one of the main concerns in the current poultry industry, causing major economic losses and affecting animal welfare. The most common bone anomalies in the femur are dyschondroplasia, femoral head separation (FHS), and bacterial chondronecrosis with osteomyelitis (BCO), also known as femoral head necrosis (FHN). The present study aimed to identify differentially expressed (DE) genes in the articular cartilage (AC) of normal and FHS-affected broilers by RNA-Seq analysis. In the transcriptome analysis, 12,169 genes were expressed in the femur AC. Of those, 107 genes were DE (FDR < 0.05) between normal and affected chickens, of which 9 were downregulated and 98 were upregulated in the affected broilers. In the gene-set enrichment analysis using the DE genes, 79 biological processes (BP) were identified and were grouped into 12 superclusters. The main BP found were involved in the response to biotic stimulus, gas transport, cellular activation, carbohydrate-derived catabolism, multi-organism regulation, immune system, muscle contraction, multi-organism process, cytolysis, leukocytes and cell adhesion. In this study, the first transcriptome analysis of the broilers femur articular cartilage was performed, and a set of candidate genes (AvBD1, AvBD2, ANK1, EPX, ADA, RHAG) that could trigger changes in the broiler´s femoral growth plate was identified. Moreover, these results could be helpful to better understand FHN in chickens and possibly in humans.
dc.language.isoeng
dc.rightsopenAccesseng
dc.subjectExpressão gênica
dc.subjectSequenciamento de RNA
dc.subjectCartilagem da cabeça do fêmur
dc.subjectSeparação da cabeça femoral
dc.subjectAnálises de expressão diferencial
dc.subjectReação da cadeia de polimerase
dc.subjectTranscriptoma da cartilagem do fêmur
dc.subjectDifferential expression analysis
dc.titleDifferentially expressed genes in the femur cartilage transcriptome clarify the understanding of femoral head separation in chickens.
dc.typeArtigo de periódico
dc.subject.thesagroFrango
dc.subject.thesagroFrango de Corte
dc.subject.nalthesaurusBroiler chickens
dc.subject.nalthesaurusGene expression
dc.subject.nalthesaurusQuantitative polymerase chain reaction
dc.subject.nalthesaurusTranscriptome
dc.description.notesArticle number: 17965.
riaa.ainfo.id1135377
riaa.ainfo.lastupdate2021-10-15
dc.identifier.doihttps://doi.org/10.1038/s41598-021-97306-3
dc.contributor.institutionLUDMILA MUDRI HUL, UNIVERSIDADE ESTADUAL DO CENTRO-OESTE, GUARAPUAVA; ADRIANA MERCIA GUARATINI IBELLI, CNPSA; IGOR RICARDO SAVOLDI, UDESC; DÉBORA ESTER PETRY MARCELINO, FACULDADE DE CONCÓRDIA; LANA TEIXEIRA FERNANDES; JANE OLIVEIRA PEIXOTO, UNIVERSIDADE ESTADUAL DO CENTRO-OESTE, GUARAPUAVA; MAURICIO EGIDIO CANTAO, CNPSA; ROBERTO HIROSHI HIGA, CNPTIA; POLIANA FERNANDA GIACHETTO, CNPTIA; LUIZ LEHMANN COUTINHO, ESALQ/USP; MONICA CORREA LEDUR, CNPSA.
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