Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1135666
Registro completo de metadados
Campo DCValorIdioma
dc.contributor.authorCOTTA, S. P. M.
dc.contributor.authorMARINS, M. S.
dc.contributor.authorMARRIEL, I. E.
dc.contributor.authorLANA, U. G. de P.
dc.contributor.authorGOMES, E. A.
dc.contributor.authorFIGUEIREDO, J. E. F.
dc.contributor.authorOLIVEIRA-PAIVA, C. A.
dc.date.accessioned2022-02-03T13:00:39Z-
dc.date.available2022-02-03T13:00:39Z-
dc.date.created2021-10-28
dc.date.issued2023
dc.identifier.citationBrazilian Journal of Biology, v. 83, e244205, 2023.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1135666-
dc.descriptionOrgano-mineral fertilizers supplemented with biological additives are an alternative to chemical fertilizers. In this study, thermoresistant microorganisms from composting mass were isolated by two-step procedures. First, samples taken at different time points and temperatures (33 days at 52 ºC, 60 days at 63 ºC, and over 365 days at 26 ºC) were pre-incubated at 80o C for 30 minutes. Second, the microbial selection by in vitro culture-based methods and heat shock at 60o C and 100o C for 2h and 4h. Forty-one isolates were able to grow at 60 °C for 4h; twenty-seven at 100 °C for 2h, and two at 100 °C for 4h. The molecular identification by partial sequencing of the 16S ribosomal gene using universal primers revealed that thirty-five isolates were from eight Bacillus species, one Brevibacillus borstelensis, three Streptomyces thermogriseus, and two fungi (Thermomyces lanuginosus and T. dupontii). Data from amylase, phytase, and cellulase activity assays and the enzymatic index (EI) showed that 38 of 41 thermo-resistant isolates produce at least one enzyme. For amylase activity, the highest EI value was observed in Bacillus licheniformis (isolate 21C2, EI= 4.11), followed by Brevibacillus borstelensis (isolate 6C2, EI= 3.66), Bacillus cereus (isolate 18C2, EI= 3.52), and Bacillus paralicheniformis (isolate 20C2, EI= 3.34). For phytase, the highest EI values were observed for Bacillus cereus (isolate 18C2, EI= 2.30) and Bacillus licheniformis (isolate 3C1, EI= 2.15). Concerning cellulose production, B. altitudinis (isolate 6C1) was the most efficient (EI= 6.40), followed by three Bacillus subtilis (isolates 9C1, 16C2, and 19C2) with EI values of 5.66, 5.84, and 5.88, respectively, and one B. pumilus (isolate 27C2, EI= 5.78). The selected microorganisms are potentially useful as a biological additive in organo-mineral fertilizers and other biotechnological processes.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectMicrorganismo termoresistente
dc.titleThermo-resistant enzyme-producing microorganisms isolated from composting.
dc.typeArtigo de periódico
dc.subject.thesagroMicrorganismo
dc.subject.thesagroFertilizante
dc.subject.thesagroEnzima
dc.description.notesPublicado em: 27 mar. 2021.
riaa.ainfo.id1135666
riaa.ainfo.lastupdate2022-02-03
dc.contributor.institutionCentro Universitário de Sete Lagoas, UNIFEMM; Universidade Federal de Lavras; IVANILDO EVODIO MARRIEL, CNPMS; UBIRACI GOMES DE PAULA LANA, CNPMS; ELIANE APARECIDA GOMES, CNPMS; JOSE EDSON FONTES FIGUEIREDO, CNPMS; CHRISTIANE ABREU DE OLIVEIRA PAIVA, CNPMS.
Aparece nas coleções:Artigo em periódico indexado (CNPMS)

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
Thermo-resistant.pdf580,36 kBAdobe PDFThumbnail
Visualizar/Abrir
Mostrar registro simples do item Visualizar estatísticas

FacebookTwitterDeliciousLinkedInGoogle BookmarksMySpace