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| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.contributor.author | BEZERRA, Y. C. dos A. | |
| dc.contributor.author | MARQUES, J. P. R. | |
| dc.contributor.author | STIPP, L. C. L. | |
| dc.contributor.author | ATTÍLIO, L. B. | |
| dc.contributor.author | ASTUA, J. de F. | |
| dc.contributor.author | MOURÃO FILHO, F. de A. A. | |
| dc.date.accessioned | 2021-11-17T12:00:36Z | - |
| dc.date.available | 2021-11-17T12:00:36Z | - |
| dc.date.created | 2021-11-17 | |
| dc.date.issued | 2021 | |
| dc.identifier.citation | Revista Brasileira de Fruticultura, Jaboticabal, v. 43, n. 4,e-005, 2021. | |
| dc.identifier.issn | 1806-9967 | |
| dc.identifier.uri | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1136203 | - |
| dc.description | New approaches for developing disease-resistant genetically modified organisms have included specific targets for gene expression to enhance the chances for pathogen control. Gene expression driven by phloem-derived Citrus sinensis gene promoters could be evaluated and compared with the expression induced by a strong constitutive promoter in the same tissue, leading to the production of transgenic sweet oranges potentially more resistant to diseases caused by phloem-limited bacteria. ?Carrizo? citrange [Poncirus trifoliata (L.) Raf. x Citrus sinensis (L.) Osbeck] was transformed, via Agrobacterium tumefaciens, with the binary vector pCAMBIA2301 bearing the uidA gene (?-glucuronidase) driven by the CaMV35S constitutive promoter (CaMV35S::uidA) or by the CsPP2.B1 (CsPP2.B1::uidA) or by the CsVTE2 (CsVTE2::uidA) citrus promoters. In vitro regenerated shoots were grafted onto ?Rangpur? lime (C. limonia Osbeck). The genetic transformation was confirmed by Southern blot analyses. uidA gene expression was evaluated by RT-qPCR, and gene histolocalization controlled by these three promoters was accessed by X-GLUC treated stem sections. uidA gene expression exhibited by tissue-specific promoters was overall lower than from the constitutive promoter CaMV35; however, constructs driven by tissuespecific promoters may lead to expression in restricted tissues. CsPP2.B1 and CsVTE2 promoters can be considered adequate for the utilization in gene constructs aiming disease resistance. | |
| dc.language.iso | por | |
| dc.rights | openAccess | pt_BR |
| dc.title | How to drive phloem gene expression? A case study with preferentially expressed citrus gene promoters. | |
| dc.type | Artigo de periódico | |
| dc.subject.thesagro | Fruta Cítrica | |
| riaa.ainfo.id | 1136203 | |
| riaa.ainfo.lastupdate | 2021-11-17 | |
| dc.contributor.institution | YANE CAROLINE DOS ANJOS BEZERRA, USP; JOÃO PAULO RODRIGUES MARQUES, USP; LILIANE CRISTINA LIBORIO STIPP, USP; LÍSIA BORGES ATTÍLIO, USP; JULIANA DE FREITAS ASTUA, CNPMF; FRANCISCO DE ASSIS ALVES MOURÃO FILHO, USP. | |
| Aparece en las colecciones: | Artigo em periódico indexado (CNPMF)  | |
Ficheros en este ítem:
| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| How-to-drive-phloem-gene-expression.pdf | 3.3 MB | Adobe PDF |  Visualizar/Abrir |
