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dc.contributor.authorGIGLIOTI, R.eng
dc.contributor.authorOKINO, C. H.eng
dc.contributor.authorAZEVEDO, B. T.eng
dc.contributor.authorGUTMANIS, G.eng
dc.contributor.authorKATIKI, L. M.eng
dc.contributor.authorOLIVEIRA, M. C. de S.eng
dc.contributor.authorVERCESI FILHO, A. E.eng
dc.date.accessioned2021-12-06T17:00:26Z-
dc.date.available2021-12-06T17:00:26Z-
dc.date.created2021-12-06
dc.date.issued2021
dc.identifier.citationFood Chemistry: Molecular Sciences, v.3, 2021, 100055.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1137153-
dc.descriptionThe rising consumption of A2 milk and its derivatives in recent years has garnered attention from both consumers and producers, mainly due its possible health benefits, such as enhanced digestion and easier absorption. Thus, a novel real-time PCR using a combination of locked nucleic acid modified (LNA) conjugated probes was developed to genotype A1 and A2 alleles of B-casein gene (CSN2) and to detect and quantify the A1 presence in A2 samples. The limit of detection for each probe (A1 and A2) was evaluated using decreasing serial dilutions. Besides, the sensitivity of A1 allele detection in the A2 samples was also tested. The limits of detection of A1 and A2 alleles were 6 copies, while for A1 allele detection in A2 samples was 7.5 copies (1%). The LNA-probe based method was found to be rapid, robust, highly sensitive, cost effective, and can be employed as screening test to certificate the A2 dairy products.
dc.language.isoeng
dc.rightsopenAccesseng
dc.subjectB Casein
dc.subjectSensitivity
dc.subjectLNA probe
dc.subjectReal time PCR
dc.titleNovel LNA probe-based assay for the A1 and A2 identification ofB-casein gene in milk samples.
dc.typeArtigo de periódico
dc.subject.nalthesaurusAlleles
dc.format.extent26 p.
riaa.ainfo.id1137153
riaa.ainfo.lastupdate2021-12-06
dc.identifier.doihttps://doi.org/10.1016/j.fochms.2021.100055
dc.contributor.institutionRODRIGO GIGLIOTI, IZeng
dc.contributor.institutionCINTIA HIROMI OKINO, CPPSEeng
dc.contributor.institutionBIANCA TAINA AZEVEDO, IZeng
dc.contributor.institutionGUNTA GUTMANIS, IZeng
dc.contributor.institutionLUCIANA MORITA KATIKI, IZeng
dc.contributor.institutionMARCIA CRISTINA DE SENA OLIVEIRA, CPPSEeng
dc.contributor.institutionANIBAL EUGENIO VERCESI FILHO, IZ.eng
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