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dc.contributor.authorSOUZA, J. A. de
dc.contributor.authorSANTOS, M. R. A. dos
dc.date.accessioned2021-12-30T15:00:24Z-
dc.date.available2021-12-30T15:00:24Z-
dc.date.created2021-12-30
dc.date.issued2021
dc.identifier.citationInternational Journal of Development Research, v. 11, n. 12, p. 52392-52393, Dec. 2021.
dc.identifier.issn2230-9926
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1138599-
dc.descriptionA protocol for regeneration of P. hispidum through somatic embryogenesis in leaf explants is described. Under aseptic conditions, the leaves were cut into 1.0 cm2 explants. These explants were inoculated into test tubes with 10.0 mL of an Murashige and Skoog (MS) basal culture medium supplemented with 30.0 g L-1 sucrose, 6.0 g L-1 agar and a factorial combination of the growth regulators 2,4-Dichlorophenoxyacetic acid (2,4-D) and 6-Benzylaminopurine (BA), both at 0.0, 1.0, 2.0, 3.0 and 4.0 mg L-1 , totalizing 25 treatments. The pH was adjusted to 5.8 and the medium autoclaved at 121°C for 20 minutes. After 75 days, the average number of cotyledonary somatic embryos in each explant was recorded.Maximum number of 9.2 cotyledonary embryos per explant was observed with the combination of 2.0 mg L-1 BA + 2.0 mg L-1 2,4-D. The subculture of the cotyledonary embryos to a medium without growth regulators resulted in 95% conversion into plantlets, which were acclimatized with 100% survival.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectEmbriogênese somática
dc.subjectCotyledonary embryos
dc.subjectProtocolo de regeneração
dc.titleIn vitro propagation of Piper hispidum by somatic embryogenesis in leaf explants.
dc.typeArtigo de periódico
dc.subject.thesagroReprodução Vegetal
dc.subject.thesagroPropagação Vegetativa
dc.subject.thesagroCultura In Vitro
dc.subject.thesagroCultura de Tecido
dc.subject.thesagroFolha
dc.subject.thesagroExplante
dc.subject.nalthesaurusPiper hispidum
dc.subject.nalthesaurusPlant reproduction
dc.subject.nalthesaurusVegetative propagation
dc.subject.nalthesaurusIn vitro culture
dc.subject.nalthesaurusTissue culture
dc.subject.nalthesaurusSomatic embryogenesis
dc.subject.nalthesaurusLeaves
dc.subject.nalthesaurusExplants
dc.subject.nalthesaurusGenetic techniques and protocols
riaa.ainfo.id1138599
riaa.ainfo.lastupdate2021-12-30
dc.identifier.doihttps://doi.org/10.37118/ijdr.23567.12.2021
dc.contributor.institutionJAQUELINE ANDRUCHEVITZ DE SOUZA, CENTRO UNIVERSITÁRIO APARÍCIO CARVALHO
dc.contributor.institutionMAURICIO REGINALDO ALVES DOS SANTOS, CPAF-RO.eng
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