Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1138600
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dc.contributor.authorSOUZA, J. A. de
dc.contributor.authorSANTOS, M. R. A. dos
dc.date.accessioned2021-12-30T15:00:38Z-
dc.date.available2021-12-30T15:00:38Z-
dc.date.created2021-12-30
dc.date.issued2021
dc.identifier.citationInternational Journal of Development Research, v. 11, n. 12, p. 52440-52442, Dec. 2021.
dc.identifier.issn2230-9926
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1138600-
dc.descriptionPiper marginatum, popularly known in Brazil as Caapeba, is a native Amazonian plant of shrubby size, traditionally used for its active principles that act as therapeutic agents in wounds and pathologies. The objective of this work was to establish a protocol for the in vitro propagation of this species from leaf explants. For this, leaves were collected from two-year-old plants kept in a greenhouse at Embrapa Rondônia, in Porto Velho, Brazil. The leaves were subjected to disinfestation in a laminar flow chamber by immersion in 70% alcohol for one minute, followed by immersion in 2.0% sodium hypochlorite with 1.0 mL of Tween 20® for five minutes. Leaves were cut into 1.0 cm2 explants, which were inoculated into MS medium supplemented with 30.0 g L-1 sucrose, 6.0 g L-1 agar and variable concentrations of growth regulators benzylaminopurine (BA) (0.0, 1.13, 2.25 and 4.50 mg L-1) and 2,4-dichlorophenoxyacetic acid (2,4-D) (0.0, 1.11, 2.21 and 4.42 mg L-1) in factorial combination. The cultures were kept in a growth room at 25°C, under a 16-hour photoperiod. After 30 days, the number of shoots formed in the explants was evaluated. These shoots were subcultured on MS medium, under the conditions described, but without growth regulators. After 60 days, plantlet height, number of leaves, leaf area and root volume were evaluated. The treatment that contained 1.13 mg L-1 of BAP, in the absence of 2,4-D, resulted in the highest number of shoots and more vigorous plantlets after subculture, in relation to all evaluated variables. The plantlets were acclimatized in a greenhouse, with 50% shading and sprinkler irrigation three times a day, for their conversion into plants.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectPiper marginatum
dc.subjectProtocolo de propagação
dc.titleDirect organogenesis and in vitro regeneration of plants of Piper marginatum.
dc.typeArtigo de periódico
dc.subject.thesagroCaapeba
dc.subject.thesagroPlanta Medicinal
dc.subject.thesagroReprodução Vegetal
dc.subject.thesagroPropagação Vegetativa
dc.subject.thesagroOrganogénese
dc.subject.thesagroRegeneração
dc.subject.thesagroCultura In Vitro
dc.subject.thesagroFolha
dc.subject.thesagroExplante
dc.subject.nalthesaurusMedicinal plants
dc.subject.nalthesaurusPlant reproduction
dc.subject.nalthesaurusVegetative propagation
dc.subject.nalthesaurusOrganogenesis
dc.subject.nalthesaurusIn vitro regeneration
dc.subject.nalthesaurusLeaves
dc.subject.nalthesaurusExplants
dc.subject.nalthesaurusGenetic techniques and protocols
riaa.ainfo.id1138600
riaa.ainfo.lastupdate2021-12-30
dc.identifier.doihttps://doi.org/10.37118/ijdr.23568.12.2021
dc.contributor.institutionJAQUELINE ANDRUCHEVITZ DE SOUZA, FIMCA
dc.contributor.institutionMAURICIO REGINALDO ALVES DOS SANTOS, CPAF-RO.eng
Appears in Collections:Artigo em periódico indexado (CPAF-RO)

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